지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충남대학교 농업과학연구소 Korean Journal of Agricultural Science Korean Journal of Agricultural Science Vol.47 No.1
- 발행연도
- 2020.3
- 수록면
- 173 - 182 (10page)
이용수
초록· 키워드
오류제보하기
Human Astrovirus (HuAstV), known as a waterborne virus, is a group IV positive-sense singlestranded RNA that belongs to Astroviridae. The first outbreak of HuAstV was reported in England in 1975. HuAstV can exist not only among clinical patients but also in various water environments, such as water for agriculture and vegetables. For diagnosis of HuAstV from water samples, a polymerase chain reaction (PCR) system has been developed. However, the PCR-based diagnostic method has problems in field application, such as reaction time, sensitivity and specificity. For this reason, in this study we developed the loop-mediated isothermal amplification assay (LAMP) system, aimed specifically at HuAstV. Three prepared LAMP primer sets were tested by specificity, non-specificity and sensitivity; one LAMP primer set was selected with optimum reaction temperature. The developed LAMP primer set reaction conditions were confirmed at 62℃, and detection sensitivity was 1 fg/μL. In addition, restriction enzyme HaeIII (GG/CC) was introduced to confirm that the LAMP reaction was positive. As a result, selected LAMP primer set was 100 - 1000 times more specific, rapid, and sensitive than conventional-nested PCR methods. For verification of the developed LAMP assay, twenty samples of cDNA from groundwater samples were tested. We expect that the developed LAMP assay will be used to diagnose HuAstV from various samples.
목차
Abstract
Introduction
Materials and Methods
Results and Discussion
Conclusion
References
참고문헌 (26)
참고문헌 신청
Brown DW / 2008 / A DNA oligonucleotide microarray for detecting human astrovirus serotypes / Journal of Virological Methods 147:86~92
Cortez V / 2017 / Astrovirus biology and pathogenesis / Annual Review of Virology 4:327~348
Eric CM / 2007 / A typical norovirus epidemic in Hong Kong during summer of 2006 caused by a new genogroup II/4 variant / Journal of Clinical Microbiology 45:2205~2011
Finkbeiner SR / 2009 / Human stool contains a previously unrecognized diversity of novel astroviruses / Virology Journal 6:161
Finkbeiner SR / 2008 / Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea / Virology Journal 5:117
Cortez V / 2017 / Astrovirus biology and pathogenesis / Annual Review of Virology 4:327~348
Eric CM / 2007 / A typical norovirus epidemic in Hong Kong during summer of 2006 caused by a new genogroup II/4 variant / Journal of Clinical Microbiology 45:2205~2011
Finkbeiner SR / 2009 / Human stool contains a previously unrecognized diversity of novel astroviruses / Virology Journal 6:161
Finkbeiner SR / 2008 / Complete genome sequence of a highly divergent astrovirus isolated from a child with acute diarrhea / Virology Journal 5:117
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UCI(KEPA) : I410-ECN-0101-2020-520-000607091