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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 536 - 545 (10page)
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초록· 키워드
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Cytochrome P450 (P450 or CYP) is involved in the metabolism of endogenous and exogenous compounds in most organisms. P450s have great potential as biocatalysts in the pharmaceutical and fine chemical industries because they catalyze diverse oxidative reactions using a wide range of substrates. The high-cost nicotinamide cofactor, NADPH, is essential for P450 reactions. Glucose-6-phosphate dehydrogenase (G6PDH) has been commonly used in NADPH-generating systems (NGSs) to provide NADPH for P450 reactions. Currently, only two G6PDHs from Leuconostoc mesenteroides and Saccharomyces cerevisiae can be obtained commercially. To supply high-cost G6PDH cost-effectively, we cloned the cytosolic G6PDH gene of Solanum lycopersicum (tomato) with 6xHis tag, expressed it in Escherichia coli, and purified the recombinant G6PDH (His-G6PDH) using affinity chromatography. In addition, enzymatic properties of His-G6PDH were investigated, and the His-G6PDH-coupled NGS was optimized for P450 reactions. His-G6PDH supported CYP102A1-catalyzed hydroxylation of omeprazole and testosterone by NADPH generation. This result suggests that tomato His-G6PDH could be a cost-effective enzyme source for NGSs for P450-catalyzed reactions as well as other NADPH-requiring reactions.
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참고문헌 (51)
참고문헌 신청
Guengerich FP / 2001 / Common and uncommon cytochrome P450 reactions related to metabolism and chemical toxicity / Chem. Res. Toxicol 14:611~650
Guengerich FP / 2013 / Unusual cytochrome P450enzymes and reactions / J. Biol. Chem 288:17065~17073
Guengerich FP / 2008 / Cytochrome P450 and chemical toxicology / Chem. Res. Toxicol 21:70~83
Tang W / 2001 / Heterotropic cooperativity of cytochrome P450 3A4 and potential drug-drug interactions / Curr. Drug Metab 2:185~198
Munro AW / 1996 / Probing electron transfer in flavocytochrome P-450 BM3 and its component domains / Eur. J. Biochem 239:403~409
Guengerich FP / 2013 / Unusual cytochrome P450enzymes and reactions / J. Biol. Chem 288:17065~17073
Guengerich FP / 2008 / Cytochrome P450 and chemical toxicology / Chem. Res. Toxicol 21:70~83
Tang W / 2001 / Heterotropic cooperativity of cytochrome P450 3A4 and potential drug-drug interactions / Curr. Drug Metab 2:185~198
Munro AW / 1996 / Probing electron transfer in flavocytochrome P-450 BM3 and its component domains / Eur. J. Biochem 239:403~409
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