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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제8호
- 발행연도
- 2018.1
- 수록면
- 1,346 - 1,351 (6page)
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초록· 키워드
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Oxidoreductases are effective biocatalysts, but their practical use is limited by the need for large quantities of NAD(P)H. In this study, a whole-cell biocatalyst for NAD(P)H cofactor regeneration was developed using the economical substrate glycerol. This cofactor regeneration system employs permeabilized Escherichia coli cells in which the glpD and gldA genes were deleted and the gpsA gene, which encodes NAD(P)+-dependent glycerol-3- phosphate dehydrogenase, was overexpressed. These manipulations were applied to block a side reaction (i.e., the conversion of glycerol to dihydroxyacetone) and to switch the glpDencoding enzyme reaction to a gpsA-encoding enzyme reaction that generates both NADH and NADPH. We demonstrated the performance of the cofactor regeneration system using a lactate dehydrogenase reaction as a coupling reaction model. The developed biocatalyst involves an economical substrate, bifunctional regeneration of NAD(P)H, and simple reaction conditions as well as a stable environment for enzymes, and is thus applicable to a variety of oxidoreductase reactions requiring NAD(P)H regeneration.
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참고문헌 (19)
참고문헌 신청
Uppada V / 2014 / Cofactor regeneration; an important aspect of biocatalysis / Curr. Sci. 106:946~957
Wang X / 2017 / Cofactor NAD(P)H regeneration inspired by heterogeneous pathways / Chem 2:621~654
Stewart JD / 2001 / Dehydrogenases and transaminases in asymmetric synthesis / Curr. Opin. Chem. Biol. 5:120~129
Li Z / 2002 / Oxidative biotransformations using oxygenases / Curr. Opin. Chem. Biol. 6:136~144
Hummel W / 1989 / Dehydrogenases for the synthesis of chiral compounds / Eur. J. Biochem. 184:1~13
Wang X / 2017 / Cofactor NAD(P)H regeneration inspired by heterogeneous pathways / Chem 2:621~654
Stewart JD / 2001 / Dehydrogenases and transaminases in asymmetric synthesis / Curr. Opin. Chem. Biol. 5:120~129
Li Z / 2002 / Oxidative biotransformations using oxygenases / Curr. Opin. Chem. Biol. 6:136~144
Hummel W / 1989 / Dehydrogenases for the synthesis of chiral compounds / Eur. J. Biochem. 184:1~13
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