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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제1호
- 발행연도
- 2020.1
- 수록면
- 93 - 100 (8page)
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A bacterial strain inhibiting the growth of Vibrio anguillarum, the causative agent of vibriosis, was isolated from fish intestines. The isolated strain HS36 was identified as Aerococcus urinaeequi based on the characteristics of the genus according to Bergey’s Manual of Systematic Bacteriology and by 16S rRNA sequencing. The growth rate and antibacterial activity of strain HS36 in shaking culture were higher than those in static culture, while the optimal pH and temperature for antibacterial activity were 7.0 and 30°C, respectively. The active antibacterial substance was purified from a culture broth of A. urinaeequi HS36 by Sephadex G-75 gel chromatography, Sephadex G-25 gel chromatography, and reverse-phase high-performance liquid chromatography. Its molecular weight, as estimated by Tricine SDS-polyacrylamide gel electrophoresis, was approximately 1,000 Da. The antibacterial substance produced by strain HS36 was stable after incubation for 1 h at 100°C. Although its antibacterial activity was optimal at pH 6-8, activity was retained at a pH range from 2 to 11. The purified antibacterial substance was inactivated by proteinase K, papain, and β-amylase treatment. The newly purified antibacterial substance, classified as a class II bacteriocin, inhibited the growth of Klebsiella pneumoniae, Salmonella enterica, and Vibrio alginolyticus.
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참고문헌 (30)
참고문헌 신청
Cordero H / 2014 / Use of probiotic bacteria against bacterial and viral infections in shellfish and fish aquaculture / Intech. Open Sci 8:239~265
Abarike ED / 2018 / Influence of traditional Chinese medicine and Bacillus species(TCMBS)on growth, immune response and disease resistance in Nile tilapia, Oreochromis niloticus / Aquac. Res 49:2366~2375
Baker-Austin C / 2018 / Vibrio spp. infections / Nat. Rev. Dis. Primers 4:8
Chatterjee S / 2012 / Vibrio related diseases in aquaculture and development of rapid and accurate identification methods / J. Marine Sci. Res. Dev. 1
Amalina NZ / 2019 / Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia
Abarike ED / 2018 / Influence of traditional Chinese medicine and Bacillus species(TCMBS)on growth, immune response and disease resistance in Nile tilapia, Oreochromis niloticus / Aquac. Res 49:2366~2375
Baker-Austin C / 2018 / Vibrio spp. infections / Nat. Rev. Dis. Primers 4:8
Chatterjee S / 2012 / Vibrio related diseases in aquaculture and development of rapid and accurate identification methods / J. Marine Sci. Res. Dev. 1
Amalina NZ / 2019 / Recent update on the prevalence of Vibrio species among cultured grouper in Peninsular Malaysia
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