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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제6호
- 발행연도
- 2020.1
- 수록면
- 937 - 945 (9page)
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초록· 키워드
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N-acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) plays a major role in development of biofilms, which contribute to rise in infections and biofouling in water-related industries. Interference in QS, called quorum quenching (QQ), has recieved a lot of attention in recent years. Rhodococcus spp. are known to have prominent quorum quenching activity and in previous reports it was suggested that this genus possesses multiple QQ enzymes, but only one gene, qsdA, which encodes an AHL-lactonase belonging to phosphotriesterase family, has been identified. Therefore, we conducted a whole genome sequencing and analysis of Rhodococcus sp. BH4 isolated from a wastewater treatment plant. The sequencing revealed another gene encoding a QQ enzyme (named jydB) that exhibited a high AHL degrading activity. This QQ enzyme had a 46% amino acid sequence similarity with the AHL-lactonase (AidH) of Ochrobactrum sp. T63. HPLC analysis and AHL restoration experiments by acidification revealed that the jydB gene encodes an AHL-lactonase which shares the known characteristics of the α/β hydrolase family. Purified recombinant JydB demonstrated a high hydrolytic activity against various AHLs. Kinetic analysis of JydB revealed a high catalytic efficiency (kcat/KM) against C4-HSL and 3-oxo-C6 HSL, ranging from 1.88 x 106 to 1.45 x 106 M-1 s-1, with distinctly low KM values (0.16-0.24 mM). This study affirms that the AHL degrading activity and biofilm inhibition ability of Rhodococcus sp. BH4 may be due to the presence of multiple quorum quenching enzymes, including two types of AHL-lactonases, in addition to AHL-acylase and oxidoreductase, for which the genes have yet to be described.
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참고문헌 (49)
참고문헌 신청
Miller M / 2001 / Quorum sensing in bacteria / Annu. Rev. Microbiol 55:165~199
Whitehead N / 2001 / Quorum-sensing in Gram-negative bacteria / FEMS Microbiol. Rev 25:365~404
Davies D / 1998 / The involvement of cell-to-cell signals in the development of a bacterial biofilm / Science 280:295~298
Dickschat J / 2010 / Quorum sensing and bacterial biofilms / Nat. Prod. Rep 27:343
Grandclément C / 2015 / Quorum quenching : role in nature and applied developments / FEMS Microbiol. Rev 40:86~116
Whitehead N / 2001 / Quorum-sensing in Gram-negative bacteria / FEMS Microbiol. Rev 25:365~404
Davies D / 1998 / The involvement of cell-to-cell signals in the development of a bacterial biofilm / Science 280:295~298
Dickschat J / 2010 / Quorum sensing and bacterial biofilms / Nat. Prod. Rep 27:343
Grandclément C / 2015 / Quorum quenching : role in nature and applied developments / FEMS Microbiol. Rev 40:86~116
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