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- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
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The functional peptides from protein hydrolysates of various fishery sources have been identified such as antioxidant activity. The main intention of this study was purification and characterization of antioxidative peptide from black eelpout muscle. The antioxidative peptides were purified from black eelpout (Lycodes diapterus) muscle using different proteases. Antioxidant activity of black eelpout hydrolysates was evaluated using DPPH radical scavenging activity. Among six hydrolysates, the pepsin hydrolysate had the highest antioxidant activity compared to the other hydrolysates. Therefore, it was further purified and a peptide with seven amino acid residues of DLVKVEA (784 Da) was identified by amino acid sequence analysis. The EC50 value for scavenging DPPH radicals by purified peptide was 688.77 μM. Additionally, the purified peptide exhibited protective effect against DNA damage induces by oxidation in mouse macrophages (RAW 264.7 cells). The results of this study suggest that black eelpout muscle protein hydrolysate could potentially contribute to development of bioactive peptides in basic research.
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참고문헌 (41)
참고문헌 신청
Racheal Abuine / 2019 / Biological activity of peptides purified from fish skin hydrolysates / Fisheries and Aquatic Sciences 22(2):1~14
Association of Official Analytical Chemist (AOAC) / 2000 / Official methods of analysis
Becker GL / 1993 / Preserving food and health : antioxidants make functional, nutritious preservatives / Food Process 12:54~56
Bougatef A / 2010 / Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle(Sardinella aurita)by-products proteins / Food Chem 118:559~565
Byun HG / 2009 / Antioxidant peptides isolated from the marine rotifer, Brachionus rotundiformis / Process Biochem 44:842~846
Association of Official Analytical Chemist (AOAC) / 2000 / Official methods of analysis
Becker GL / 1993 / Preserving food and health : antioxidants make functional, nutritious preservatives / Food Process 12:54~56
Bougatef A / 2010 / Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle(Sardinella aurita)by-products proteins / Food Chem 118:559~565
Byun HG / 2009 / Antioxidant peptides isolated from the marine rotifer, Brachionus rotundiformis / Process Biochem 44:842~846
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