지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Salmonella is one of the most important bacterial pathogens responsible for many zoonotic food-related infectious diseases. Quantitative detection of the foodborne Salmonella contamination in various food sources is therefore critical for preventing the related disease outbreaks. In this study, we developed and evaluated a reliable real-time polymerase chain reaction (RT-PCR) assay to detect the Salmonella contamination quantitatively. The experimental results showed that our invA gene-specific quantitative RT-PCR (qRT-PCR) assay provides a strong correlation between the Cq values and the direct plate counts of Salmonella species in the artificially formulated samples. Further study may be necessary to identify more accurate correlation and equation that can apply to Salmonella spp.
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참고문헌 (12)
참고문헌 신청
Ahmed OB / 2014 / Detection of Salmonella in food samples by culture and polymerase chain reaction methods / J Bacteriol Parasitol 5(3):187
Barbau-Piednoir E / 2013 / SYBR® Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels / Appl Microbiol Biotechnol 97(22):9811~9824
Centers for Disease Control and Prevention (CDC) / 2019 / Surveillance for Foodborne Disease Outbreaks, United States, 2017
Chung YH / 2003 / Prevalence and antibiotic susceptibility of Salmonella isolated from foods in Korea from 1993 to 2001 / J Food Prot 66(7):1154~1157
Jasson V / 2010 / Alternative microbial methods : An overview and selection criteria / Food Microbiol 27(6):710~730
Barbau-Piednoir E / 2013 / SYBR® Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels / Appl Microbiol Biotechnol 97(22):9811~9824
Centers for Disease Control and Prevention (CDC) / 2019 / Surveillance for Foodborne Disease Outbreaks, United States, 2017
Chung YH / 2003 / Prevalence and antibiotic susceptibility of Salmonella isolated from foods in Korea from 1993 to 2001 / J Food Prot 66(7):1154~1157
Jasson V / 2010 / Alternative microbial methods : An overview and selection criteria / Food Microbiol 27(6):710~730
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