지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.1
- 수록면
- 105 - 110 (6page)
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초록· 키워드
오류제보하기
A total of 41 Salmonella (S.) strains were isolated from pigs suffered with severe watery diarrhea and were tried to identify by both matrix-assisted laser desorption ionization-time of flight mass spectrome-try (MALDI-TOF MS) and polymerase chain reaction (PCR) analysis. Fibrinous exudate and ulceration in the large intestine were prevalent in gross observation, and variable degrees of enteritis were observed in the histology of large intestines. Subsequent polymerase chain reaction (PCR) analyses demonstrated that 41 strains were identified as S. Typhimurium (39 strains), though 2 stains were failed to identify. Further identification was performed using both direct smear and protein extraction method by MALDI-TOF MS analyses. In terms of extraction methods, 100% (41/41) of isolates were identified to species level of S. spp. Whereas only 43.9% (18/41) were identified to species level using the direct method. These results thus suggest that rapid and accurate diagnosis of porcine salmonellosis can be guaranteed by MALDI-TOF MS combined with protein extraction method.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Abubakar I / 2007 / A systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food / Health Technol Assess 11:1~216
Anderson NW / 2012 / Effects of solid-medium type on routine identification of bacterial isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry / J Clin Microbiol 50:1008~1013
Baggesen DL / 1996 / Herd prevalence of Salmonella enterica infections in Danish slaughter pigs determined by microbiological testing / Prev Vet Med 26:201~213
Brown CC / 2007 / Jubb, Kennedy, and Palmer's Pathology of Domestic Animals Vol 2 / Saunders Elsevier :193~199
Chiu TH / 2005 / Development of PCR primers for the detection of Salmonella enterica serovar Choleraesuis based on the fliC gene / J Food Prot 68:1575~1580
Anderson NW / 2012 / Effects of solid-medium type on routine identification of bacterial isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry / J Clin Microbiol 50:1008~1013
Baggesen DL / 1996 / Herd prevalence of Salmonella enterica infections in Danish slaughter pigs determined by microbiological testing / Prev Vet Med 26:201~213
Brown CC / 2007 / Jubb, Kennedy, and Palmer's Pathology of Domestic Animals Vol 2 / Saunders Elsevier :193~199
Chiu TH / 2005 / Development of PCR primers for the detection of Salmonella enterica serovar Choleraesuis based on the fliC gene / J Food Prot 68:1575~1580
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