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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 69 - 77 (9page)
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The tumor suppressor gene, p53, is inactivated in the human hepatocellular carcinoma cells line, Hep3B. Berberine has been reported to inhibit the proliferation of cancer cells. This study examined whether apoptosis was induced in berberine-treated Hep3B cells and observed the association between apoptosis and the expression of p53 and DNA methyltransferase (DNMT). The cell viability was measured using an MTT assay. Apoptosis of Hep3B was measured using annexin V flow cytometry. Berberine-treated cells were examined for their DNMT enzymatic activity, mRNA expression, and protein synthesis. The p53 levels were examined by Western blot analysis. The berberine treatment resulted in increased Hep3B cell death and apoptosis in a time- and dose-dependent manner. The DNMT3b activity, mRNA expression, and protein levels all decreased after the berberine treatment. In contrast, the p53 protein levels increased with a concomitant decrease in DNMT3b. No change in the expression of ERK was observed, but the P-ERK levels decreased in a dose dependent manner. These results indicate that a treatment of Hep3B cells with berberine can reduce the expression of DNMT3b, leading to an increase in the tumor suppressant gene p53 and an increase in cell apoptosis. This shows that berberine can effectively suppress the proliferation of liver cancer cells.
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참고문헌 (39)
참고문헌 신청
Simonetti RG / 1991 / Hepatocellular carcinoma a worldwide problem and the major risk factors / Dig Dis Sci 36:962~972
Stephen B Baylin / 2000 / DNA hypermethylation in tumorigenesis: epigenetics joins genetics / Trends in Genetics 16(4):168~174
Hye-Jung Park / 2006 / DNA methyltransferase expression and DNA hypermethylation in human hepatocellular carcinoma / Cancer Letters 233(2):271~278
Bong-Kyeong Oh / 2007 / DNA methyltransferase expression and DNA methylation in human hepatocellular carcinoma and their clinicopathological correlation / International Journal of Molecular Medicine 20:65~73
Rovertson KD / 2001 / DNA methylation, methyltransferases, and cancer / Oncogene 20:3139~3155
Stephen B Baylin / 2000 / DNA hypermethylation in tumorigenesis: epigenetics joins genetics / Trends in Genetics 16(4):168~174
Hye-Jung Park / 2006 / DNA methyltransferase expression and DNA hypermethylation in human hepatocellular carcinoma / Cancer Letters 233(2):271~278
Bong-Kyeong Oh / 2007 / DNA methyltransferase expression and DNA methylation in human hepatocellular carcinoma and their clinicopathological correlation / International Journal of Molecular Medicine 20:65~73
Rovertson KD / 2001 / DNA methylation, methyltransferases, and cancer / Oncogene 20:3139~3155
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