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논문 기본 정보

자료유형
학술저널
저자정보
박영일 (서울대학교 농업생명과학대학 동물자원과학과) 임경순 (서울대학교 농업생명과학대학 동물자원과학과) 한재용 (서울대학교 농업생명과학대학 동물자원과학과) 남경우 (서울대학교 농업생명과학대학 동물자원과학과) 황규춘 (서울대학교 농업생명과학대학 동물자원과학과) 박화춘 (서울대학교 농업생명과학대학 동물자원과학과)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제20권 제2호
발행연도
1996.1
수록면
89 - 99 (11page)

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The purpose of this study was to develop the diagnosis techniques for sex determination of rabbit embryos at preimplantation stage. To detect male specific sequences using polymerase chain reaction, two genes functional on sex determination including SRY and ZFX/Y genes were targeted using multiple oligonucleotide primer sets. Three of them for conserved SRY gene were used for appropriate amplification pattern, and then only one primer set #3 proved to be most efficient, showing male-specific strong signal ofamplified sequences. Using this male specific bandsfrom human, cattle, pig and mouse, the gender of rabbit was determined. As an another system for sex determination system, amplified 910bp fragment from ZFX/Y was digested with several restriction endonuclease and showed gender specific restriction fragments only by Hinf I. Using two different system for sex identification of rabbit in this study, blind tests for 17 samples was conducted and showed identical results from two different methods. And then, amplification limit of PCR reaction for template DNA was estimated using various amounts of DNA for both SRY and ZFX/Y systems, resulted as 20pg and 800pg, respectively. With this results, test for gender identification of rabbit embryos were performed using SRY derived amplification system. From total 22 embryos selected for its developmental state 18 were identified as male embryos, showing significant difference from expected sex ratio 1:1. This biased sex ratio was interpreted as to have been caused by the fact, reported by the fact, reported by several researchers, that male embryos develop more rapidly and are more resistant against the in vitro manipulation than female embryos.

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