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논문 기본 정보

자료유형
학술저널
저자정보
박창식 (College of Agriculture, Chungnam National University) 이규승 (College of Agriculture, Chungnam National University) 박병권 (College of Agriculture, Chungnam National University) 장학규 (Tian Jin Institute of Animal Husbandry and Veterinary Science) 이의해 (Tian Jin Institute of Animal Husbandry and Veterinary Science) 서직 (Tian Jin Institute of Animal Husbandry and Veterinary Science)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제21권 제1호
발행연도
1997.1
수록면
19 - 23 (5page)

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This present study was carried out to examine the effect of maturation media and liquid boar semen on in vitro maturation and feritilization of pig oocytes. The results obtained were as follows : When the oocytes were cultured for 36∼42 hours in mTCM-199, Waymouth MB 725/1 and mTLP-PVA medium, the maturation rates were 90%, 92% and 88%, respectively. The sperm penetration rates of pig oocyte matured in vitro were 87%(mTCM-199), 90%(Waymouth MB 725/1) and 86%(mTLP-PVA), respectively. The rates of nuclear maturation and fertilization of pig oocytes among three different media did not differ. However, the rate of male pronucleus formation of pig oocytes was significantly higher in pig oocytes matured in Waymouth MB 725/1(91%) than oocytes matured in mTCM-199(66%) and mTLP-PVA(62%) medium (P<0.05). When the collected sperm-rich fraction without diluent was used fro in vitro fertilization in mTCM-199 fertilization medium, the fertilization rate was 87.9%. However, when the liquid boar semen diluted with B tschwiler diluent was used at day 3 and 5 after dilution, the fertilization rate was 40.8% and 0.0%, respectively.

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