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논문 기본 정보

자료유형
학술저널
저자정보
Hwang, Kyung-Hwa (College of Pharmacy, Chungbuk National University) Lee, Kang-Woo (College of Pharmacy, Chungbuk National University) Kim, Chang-Soo (College of Pharmacy, Chungbuk National University) Han, Kun (College of Pharmacy, Chungbuk National University) Chung, Youn-Bok (College of Pharmacy, Chungbuk National University) Moon, Dong-Cheul (College of Pharmacy, Chungbuk National University)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제24권 제6호
발행연도
2001.1
수록면
601 - 606 (6page)

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A simple assay method of recombinant human epidermal growth factor (rhEGF) in a pharmaceutical preparation was studied and validated by capillary electrophoresis (CE) using micellar electrokinetic chromatography (MEKC) techniques. Factors affecting the migration behavior and separation performances of the peptide; type of buffers pH, butler concentration, and concentration of sodium dodecyl sulfates (SDS) were investigated to optimize the analytical performance. CE was performed using running buffers 50.0 mM borate (pH 8.5) containing 12.5 mM SDS at 20 $mutextrm{V}$ of the applied voltage. Calibration curves for the rhEGF showed good linearity (r>0.999) over the wide dynamic range from 1.25 to $100{\mu\textrm{g}}/ml$. Sample analysis was performed by using standard addition method to eliminate the matrix effects of dosage vehicle. This method is assumed to be useful for quality control (QC) of various forms of pharmaceutical products of the peptide.

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