EST analysis of regenerating newt retina

Hisatomi, Osamu; Hasegawa, Akiyuki; Goto, Tatsushi; Yamamoto, Shintaro; Sakami, Sanae; Kobayashi, Yuko; Tokunaga, Fumio

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자료유형: 학술저널

저자정보: Hisatomi, Osamu (Department of Earth and Space Science, Graduate School of Science, Osaka University) Hasegawa, Akiyuki (Department of Earth and Space Science, Graduate School of Science, Osaka University) Goto, Tatsushi (Department of Earth and Space Science, Graduate School of Science, Osaka University) Yamamoto, Shintaro (Department of Earth and Space Science, Graduate School of Science, Osaka University) Sakami, Sanae (Department of Earth and Space Science, Graduate School of Science, Osaka University) Kobayashi, Yuko (Department of Earth and Space Science, Graduate School of Science, Osaka University) Tokunaga, Fumio (Department of Earth and Space Science, Graduate School of Science, Osaka University)

저널정보: 한국광과학회 Journal of photoscience : an international journal officail organ of the korean society of photoscience Journal of photoscience : an international journal officail organ of the korean society of photoscience 제9권 제2호

발행연도: 2002.1

수록면: 267 - 268 (2page)

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A vertebrate retina is an organ belonging to the central nerve system (CNS), and is usually difficult to regenerate except at an embryonic stage in life. However, certain species of urodele amphibians, such as newts and salamanders, possess the ability to regenerate a functional retina from retinal pigment epithelial (RPE) cells even as adults. After surgical removal of neural retinas from adult newt eyes, the remaining RPE cells lose their pigment granules, transdifferentiate into retinal progenitor cells, which further differentiate into various retinal neurons, and then finally reform a functional neural network. To understand the molecular mechanisms of CNS regeneration, we attempted to investigate the genes expressing in regenerating newt retina. mRNAs were isolated from regenerating retinas at 18-19 days after the surgical removal of the normal retina, and a cDNA library (regenerating retinal cDNA library) were constructed. Our EST analysis of 112 clones in the regenerating cDNA library revealed that about 70% clones are closely related to the genes previously identified. About 40% clones are housekeeping genes, and about 15% clones encode proteins related to the regulation of gene expression and to the proliferation of the cells. Sequences similar to neural retina- and RPE-specific genes were not detected at all. These results led us to suppose that the regenerating retinal cells are in a state considerably different from those of neither neural retina nor RPE cells.

#Retina #Regeneration #Differentiation #Expression sequence tag #Newt #Cynops pyrrhogaster)

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