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자료유형
학술저널
저자정보
Hong, In-Pyo (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, R.D.A.) Kim, Han-Kyoung (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, R.D.A.) Park, Jeong-Sik (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, R.D.A.) Kim, Gwang-Po (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, R.D.A.) Lee, Min-Woong (Department of Applied Biology, Dongguk University) Guo, Shun-Xing (Institute of Medicinal Plant, Chinese Academy of Medical Sciences)
저널정보
한국균학회 Mycobiology Mycobiology 제30권 제1호
발행연도
2002.1
수록면
22 - 26 (5page)

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This study was carried out to investigate the possibility for seeds germination of Gastrodia elata using symbiotic fungi. Since seeds of G. elata are very small and lack an endosperm and other nutrients, their germination is difficult without requirement for external nutrients. Out of twenty six isolates collected from protocorms of G. elata and roots of native orchids inhabited in wild, two strains(H-2 and H-21) were observed to stimulate the seed germination of G. elata. The seed germination of G. elates was excellent on oak tree leaves medium. The optimal conditions for mycelial growth of symbiotic fungi were $25^{\circ}C$ and pH 6.0, respectively. The mycelial growth of H-2 strain was excellent on YMA medium, while H-21 was poor on PDA medium. In case of carbon sources, the mycelial growth of H-2 and H-21 was good on media supplemented with glucose and dextrin, respectively. Calcium nitrate was good for mycelial growth of H-2 strain as a nitrogen sources, whereas urea was effective to H-21 strain.

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