Preparation and Gene Delivery of Alkaline Amino Acids-Based Cationic Liposomes

Li, Li; Nie, Yu; Zhu, Rong; Shi, Sanjun; Luo, Kui; He, Bin; Yang, Yang; Yang, Junxiao; Gu, Zhongwei

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자료유형: 학술저널

저자정보: Li, Li (National Engineering Research Center for Biomaterials, Sichuan University) Nie, Yu (National Engineering Research Center for Biomaterials, Sichuan University) Zhu, Rong (National Engineering Research Center for Biomaterials, Sichuan University) Shi, Sanjun (National Engineering Research Center for Biomaterials, Sichuan University) Luo, Kui (National Engineering Research Center for Biomaterials, Sichuan University) He, Bin (National Engineering Research Center for Biomaterials, Sichuan University) Yang, Yang (National Engineering Research Center for Biomaterials, Sichuan University) Yang, Junxiao (National Engineering Research Center for Biomaterials, Sichuan University) Gu, Zhongwei (National Engineering Research Center for Biomaterials, Sichuan University)

저널정보: 대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제31권 제7호

발행연도: 2008.1

수록면: 924 - 931 (8page)

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Cationic lipids 1, 2, and 3, based on hydrophobic cholesterol linked to L-lysine, L-histidine or Larginine, respectively, were designed and tested as gene delivery vectors. Physicochemical and biological properties of all liposomes and lipoplexes were evaluated, including lipid-DNA interactions, size, morphology, zeta potential, acid-base buffering capability, protection of DNA from DNase I digestion, and cytotoxity. The efficiency of luciferase gene transfection of lipoplexes 1-3 was compared with that of commercial dioleoyl-trimethylammonium propane (DOTAP) and polyethyleneimine (PEI) in 293T cells and HepG2 cells with or without poly(ethylene glycol) PEG stabilizer. The complexation and protection of DNA of liposome 3 was the strongest among the three liposomes. The efficiency of gene transfection of liposomes 1-3 was two-to threefold higher than that of PEI and/or DOTAP in 293T cells. Liposomes 1 and 3 in PEG as stabilizer showed sixfold higher transfection efficiency than that of PEI and/or DOTAP, whereas liposome 2 showed very low transfection efficiency. In HepG2 cells, the transfection efficiency of all the cationic liposomes was much lower than that of DOTAP. In conclusion, lipids 1-3 were efficient and non-toxic gene vectors; the headgroup of cationic lipids and the stabilizer of liposome formulation had an important influence on gene transfection.

#Cationic liposomes #Alkaline amino acids #Cholesterol #Gene delivery #Transfection efficiency

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