지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.9
- 수록면
- 187 - 194 (8page)
이용수
초록· 키워드
오류제보하기
A short peptide corresponding to the nuclear localization signal (NLS) of human immunodeficiency virus (HIV)-1 Tat protein, Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg, was employed to improve the efficiency of cellular uptake of nucleic acids. The peptide was first mixed with a reporter plasmid and then with cationic liposomes to form a tripartite complex of DNA/peptide/liposomes (DPL). Transfection efficiency of the DPL complex was compared with that of the conventional DNA/liposomes (DL) complex. When the DPL complex was formed with various cationic liposomes, DOTAP/DOPE (DP) liposome exhibited superior transfection efficiency to other liposomes tested in vitro. With the inclusion of the peptide, the DPL complex showed much enhanced transfection in various cancer cell lines. Particularly, transfection of the DPL complex in serum increased cellular uptake of a transgene up to 2 fold when compared with that in a serum free condition. Further, when the DPL complex was infused through the ureteric route of a rat, transfection efficiency was shown to be better in reporter gene expression than that obtained with the DL complex. This study shows that the DPL complex that is easy to formulate can be employed for much enhanced cellular uptake of a transgene.
목차
영어 초록
서론
재료 및 방법
결과
고찰
Acknowledgement
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Preparation and Gene Delivery of Alkaline Amino Acids-Based Cationic Liposomes
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2008 .01
-
Optomizing Transfection Efficiency of Cervical Cancer Cells Transfected by Cationic Liposomes Lipofectamine<sup>TM</sup>2000
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Gene Transfer Into Human Chondrocyte Derived Cells Using A Liposome Mediated Transfection System
대한정형외과학회지
2001 .04
-
Polyethylene glycol (PEG)-modified cationic liposome mediated gene delivery
대한약학회 학술대회
2002 .01
-
Improved Stability of Liposome by Association of Amphiphilic Polyelectrolytes
대한화장품학회지
2007 .01
-
Application of PEG-coated liposomes for oral delivery of peptide: Effect of liposome composition on the gastrointestinal absorption of rhEGF
대한약학회 학술대회
2001 .01
-
Gamma camera/MR dual imaging liposome labeled with radioisotope and paramagnetic ions
대한방사성의약품학회지
2017 .01
-
EXPERIMENTAL STUDY ON CATIONIC LIPOSOME-MEDIATED P53 GENE THERAPY
대한인터벤션영상기술학회지
2000 .04
-
Examination of alginate/PEl/DNA polyplex as a gene delivery system: enhancing transfection efficiency in the presence of serum and reducing cytotoxicity
대한약학회 학술대회
2002 .01
-
Effect of saltss on the entrapment of calf thymus DNA into liposomes
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1987 .01
-
방사성동위원소표지 Liposome의 분포에 대한 실험적 연구
대한핵의학회지
1990 .01
-
DNA Transfection in SK-N-BE(2)C Human Neuroblastoma Cells
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
1993 .01
-
Association of Leptin Receptor Lys109Arg and Gln223Arg Polymorphisms with Increased Risk of Clear Cell Renal Cell Carcinoma
Asian Pacific journal of cancer prevention : APJCP
2014 .01
-
PREPARATION AND PHARMACOKINETICS OF METHOTREXATE DELIVERY SYSTEM USING LONG-CIRCULATING LIPOSOMES
한국응용약물학회 춘계학술발표논문집
1996 .01
-
Associations Between XRCC1 Arg399Gln, Arg194Trp, and Arg280His Polymorphisms and Risk of Differentiated Thyroid Carcinoma: A Meta-analysis
Asian Pacific journal of cancer prevention : APJCP
2013 .01
-
DEVELOPMENT OF DRIED LIPOSOMES CONTAUBUBG $\beta$-GALACTO-SIDASE FOR THE DIGESTION OF LACTOSE IN MILK.
한국응용약물학회 춘계학술발표논문집
1996 .01
-
腹部 鈍傷後 施行한 診斷的 腹腔洗滌術의 意義 : 131名 對象
Annals of Surgical Treatment and Research
1987 .01
-
Enhancement of Emulsion-mediated Gene Expression by Using Chitosan as a Pre-Condensing Agent
대한약학회 학술대회
2002 .01
-
Optimization of Gene Delivery Mediated by Lipoplexes and Electroporation into Mouse Mesenchymal Stem Cells
대한의생명과학회지
2009 .12
-
Relative Bioavailability of Coenzyme Q10 in Emulsion and Liposome Formulations
Biomolecules & Therapeutics(구 응용약물학회지)
2010 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2009-510-017341691