Radiolabeling of antibody-mimetic scaffold protein with<sup> 99m</sup>Tc tricarbonyl precursor via hexahistidine (His<sub>6</sub>)-tag

Shim, Ha Eun; Kim, Do Hee; Lee, Chang Heon; Choi, Dae seong; Lee, Dong-Eun

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자료유형: 학술저널

저자정보: Shim, Ha Eun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) Kim, Do Hee (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) Lee, Chang Heon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) Choi, Dae seong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) Lee, Dong-Eun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)

저널정보: 대한방사성의약품학회 대한방사성의약품학회지 대한방사성의약품학회지 제5권 제1호

발행연도: 2019.1

수록면: 11 - 17 (7page)

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Recently, antibody-like scaffold proteins have received a great deal of interest in diagnosis and therapy applications because of their intrinsic features that are often required for tumor imaging and therapy. Intrinsic issues that are associated with therapeutic application of antibody-like scaffold proteins, particularly in cancer treatment, include an efficient and straightforward radiolabeling for understanding in vivo biodistribution and excretion route, and monitoring therapeutic responses. Herein, we report an efficient and straightforward method for radiolabeling of antibody-like scaffold proteins with the $[^{99m}Tc(OH_2)_3(CO)_3]^+$ ($^{99m}Tc$-tricarbonyl) by using a site-specific direct labeling method via hexahistidine-tag, which is a widely used for general purification of recombinant proteins with His-affinity chromatography. Repebody is a new class of antibody-like scaffold protein that consists of highly diverse leucine-rich repeat (LRR) modules. Although all possible biomedical applications with repebody are ongoing, it's in vivo biodistribution and excretion pathway has not yet been explored. In this study, hexahistidine ($His_6$)-tag bearing repebody (rEgH9) was labeled with [$^{99m}Tc$]-tricarbonyl. Repebody protein was radiolabeled with high radiolabeling efficiency (>90%) and radiolabeled compound was more than 99% pure after purification. These results clearly demonstrate that the present radiolabeling method will be useful molecular imaging study.

#Radiolabeling #$^{99m}Tc$ #tricarbonyl precursor #repebody protein #radio-ITLC

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