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논문 기본 정보

자료유형
학술저널
저자정보
Lee, Jae-Dal (Department of Animal Science, Chungbuk National University) Cui, Xiang-Shun (Department of Animal Science, Chungbuk National University) Im, Gi-Sun (National Livestock Research Institute, RDA) Seong, Hwan-Hoo (National Livestock Research Institute, RDA) Kim, Nam-Hyung (Department of Animal Science, Chungbuk National University)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제32권 제2호
발행연도
2008.1
수록면
71 - 79 (9page)

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Surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF MS) is one of the recently developed proteomic technologies which is based on capturing proteins and peptides by chemically modified surfaces and highly sensitive for the analysis of complex biological samples. In the present study, to gain insights into oocyte maturation and early embryo development, SELDI-TOF-MS was used to find the protein candidates that are specifically or prominently expressed in porcine oocytes at the in vitro matured metaphase II (MIIl) and germinal vesicle (GV) stages. By selected CM10 chip, 16 candidates were found to be up-regulated in GV stage oocytes compared with in MII stage oocytes, their molecular weights were 8,180 (2 candidates), 10,226 (5 candidates), 15,767 (5 candidates) and 16,770 (4 candidates) Da respectively. And the expression of 29 candidates were higher in MII than in GV stage oocytes, their molecular weight were 10,832 (3 candidates), 17,743 (8 candidates), 20,122 (3 candidates), 22,131 (3 candidates), 24,857 (7 candidates) and 33,507 (5 candidates) Da, respectively. The expression of selected 13 candidates (0.2 and 1.0 % error tolerances) were analyzed using real time RT-PCR. The proteins that differentially regulated during oocyte in vitro maturation in the pigs may be potential biomarkers of oocyte maturation and quality.

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