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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 13 - 22 (10page)
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This study aimed to develop a bile-responsive expression system for lactobacilli. The promoters of four genes, encoding phosphoenolpyruvate-dependent sugar phosphotransferase (mannose-specific), L-lactate dehydrogenase (LDH), HPr kinase, and D-alanine-D-alanine ligase, respectively, which were highly expressed by bile addition in Lactobacillus johnsonii PF01, were chosen. Each promoter was amplified by polymerase chain reaction and fused upstream of the ${\beta}$-glucuronidase gene as a reporter, respectively. Then, these constructs were cloned into E. coli-Lactobacillus shuttle vector pULP2, which was generated by the fusion of pUC19 with the L. plantarum plasmid pLP27. Finally, the constructed vectors were introduced into L. plantarum for a promoter activity assay. The LDH promoter showed the highest activity and its activity increased 1.8-fold by bile addition. The constructed vector maintained in L. plantarum until 80 generations without selection pressure. A bile-responsive expression vector, $pULP3-P_{LDH}$, for Lactobacillus spp. can be an effective tool for the bile-inducible expression of bioactive proteins in intestine after intake in the form of fermented dairy foods.
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참고문헌 (35)
참고문헌 신청
Alcantara C / 2012 / Proteomic and transcriptomic analysis of the response to bile stress of Lactobacillus casei BL23 / Microbiology 158:1206~1218
Axelsson L / 2003 / Development of an inducible gene expression system for Lactobacillus sakei / Lett Appl Microbiol 37:115~120
Benbouziane B / 2013 / Development of a Stress-Inducible Controlled Expression(SICE)system in Lactococcus lactis for the production and delivery of therapeutic molecules at mucosal surfaces / J Biotechnol 168:120~129
Bermudez-Humaran LG / 2013 / Engineering lactococci and lactobacilli for human health / Curr Opin Microbiol 16:278~283
Bohmer N / 2013 / A novel manganese starvation-inducible expression system for Lactobacillus plantarum / FEMS Microbiol Lett 342:37~44
Axelsson L / 2003 / Development of an inducible gene expression system for Lactobacillus sakei / Lett Appl Microbiol 37:115~120
Benbouziane B / 2013 / Development of a Stress-Inducible Controlled Expression(SICE)system in Lactococcus lactis for the production and delivery of therapeutic molecules at mucosal surfaces / J Biotechnol 168:120~129
Bermudez-Humaran LG / 2013 / Engineering lactococci and lactobacilli for human health / Curr Opin Microbiol 16:278~283
Bohmer N / 2013 / A novel manganese starvation-inducible expression system for Lactobacillus plantarum / FEMS Microbiol Lett 342:37~44
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