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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 25 - 31 (7page)
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Clusterin is a heterodimeric sulfated glycoprotein and plays a role in many different types of cancer as a cell survival factor and helps cancerous cells to evade stress-induced apoptosis. To investigate whether the regulation of clusterin expression is involved in the mechanism of anticancer agent, we studied the effect of tamoxifen on clusterin expression in human prostate cancer PC-3 cells. Treatment of PC-3 cells with tamoxifen reduced cellular proliferation. Western blot analyses showed that treatment with tamoxifen suppressed clusterin expression in a concentration-dependent manner. Transfection with clusterin siRNA plasmid showed that clusterin is required for PC-3 cell survival. We found that tamoxifen resulted in a rapid decrease in the phosphorylation of Akt on Ser473 leading to prevent kinase activity. Expression of myristoylated Akt prevented tamoxifen-mediated clusterin downregulation. Interestingly, MG132, a wellknown proteasome inhibitor also recovered clusterin expression suppressed by tamoxifen. These data indicate that clusterin expression may be regulated by activation of Akt and ubiquitin-proteasome pathway plays an important role in tamoxifen-mediated clusterin suppression.
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참고문헌 (37)
참고문헌 신청
Ammar H. / 2008 / Clusterin activates survival through the phosphatidylinositol 3-kinase/Akt pathway / J. Biol. Chem 283:12851~12861
Bettuzzi, S. / 2002 / Clusterin (SGP-2) transient overexpression decreases proliferation rate of SV40-immortalized human prostate epithelial cells by slowing down cell cycle progression / Oncogene 21:4328~4334
Blaschuk, O. / 1983 / Purification and characterization of a cell-aggregating factor (clusterin), the major glycoprotein in ram rete testis fluid / J. Biol. Chem 258:7714~7720
Brunet, A. / 1999 / Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor / Cell 96:857~868
Caccamo, A. E. / 2003 / Nuclear translocation of a clusterin isoform is associated with induction of anoikis in SV40-immortalized human prostate epithelial cells / Ann. N Y Acad. Sci 1010:514~519
Bettuzzi, S. / 2002 / Clusterin (SGP-2) transient overexpression decreases proliferation rate of SV40-immortalized human prostate epithelial cells by slowing down cell cycle progression / Oncogene 21:4328~4334
Blaschuk, O. / 1983 / Purification and characterization of a cell-aggregating factor (clusterin), the major glycoprotein in ram rete testis fluid / J. Biol. Chem 258:7714~7720
Brunet, A. / 1999 / Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor / Cell 96:857~868
Caccamo, A. E. / 2003 / Nuclear translocation of a clusterin isoform is associated with induction of anoikis in SV40-immortalized human prostate epithelial cells / Ann. N Y Acad. Sci 1010:514~519
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난소암 세포주에서 클러스터린의 항암제 결합과 항암제 내성의 연관성
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Expression of Clusterin in the Salivary Gland under Restraint Stress
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자궁경부암 세포주에서 Clusterin 발현과paclitaxel 내성
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자궁경부암 조직에서 Clusterin의 발현양상
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당뇨시 백서 타액선 조직내의 clusterin 변화에 관한 면역학적 연구 ( The Immunological Study On Expression of The Clusterin In Streptozotocin - Induced Diabetic Rats )
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Detection of Clusterin in Saliva on the Various Systemic Condition
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2004 .03
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스트레스에 의한 혈당변화가 타액선내 Clusterin 발현에 미치는 영향 ( Clusterin ( SGP - 2 ) In The Salivary of Insulin Injected Rats Under Stress )
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스트레스가 백서 타액선조직내의 Clusterin ( Sulfated Glycoprotein - 2 ) 변화에 미치는 영향 ( The Effect of Clusterin ( SPG - 2 ) To The Stress On The Salivary Galnds of Rats )
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상피성난소종양에서 clusterin, bax, p53, Ki-67의 발현 및 세포사멸지수에 관한 연구
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Effects of Chronic and Acute Stress on Clusterin Secretion of the Rat Submandibular Gland
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Expression of Clusterin in the Salivary Gland under Restraint Stress
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Tamoxifen Resistance in Breast Cancer
Biomolecules & Therapeutics(구 응용약물학회지)
2012 .01
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방사선조사가 백서 타액선의 clusterin 발현에 미치는 영향
대한구강악안면방사선학회지
2006 .01
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Decreased of Clusterin mRNA Expression of Epididymis Following Exposure to Bisphenol A Diglycidyl Ether during Gestation and Lactation in Sprague-Dawley Rats
Environmental Analysis Health and Toxicology
2008 .12
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스트레스가 백서 타액선조직내의 Clusterin ( Sulfated Glycoprotein - 2 ) 변화에 미치는 영향
Journal of Oral Medicine and Pain
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Effects of mixed formulation of tamoxifen and blue honeysuckle on the pharmacokinetics profiles of tamoxifen after single oral administration
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