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자료유형
학술저널
저자정보
Kwon, Yoon Kyung (Department of Food and Biotechnology, Korea University) Choi, Soo Jung (Functional Food Research Center, Korea University) Kim, Cho Rong (Functional Food Research Center, Korea University) Kim, Jae Kyeom (Department of Food Science and Nutrition, University of Minnesota) Kim, Youn-Jung (College of Nursing Science, Kyung Hee University) Choi, Jong Hun (Natural Way Co., Ltd.) Song, Sang-Wook (Department of Family Medicine, St. Vincent's Hospital, College of Medicine, The Catholic University of Korea) Kim, Chang-Ju (Department of Physiology, Kyung Hee University) Park, Gwi Gun (Department of Food Science and Biotechnology, GaChon University) Park, Cheung-Seog (School of Medicine, Department of Microbiology, Kyung Hee University) Shin, Dong-Hoon (Department of Food and Biotechnology, Korea University)
저널정보
한국응용생명화학회 Applied Biological Chemistry Applied Biological Chemistry 제59권 제4호
발행연도
2016.1
수록면
553 - 565 (13page)

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Many studies have shown that oxidative stress induced by hydrogen peroxide can lead to neuronal cell death. In this study, protective effects of the ethanol extract of A. lappa L. roots against oxidative stress in PC12 cells were measured by 2', 7'-dichlorofluorescein diacetate and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Oxidative stress contributes to the memory deficits induced by Beta-$amyloid_{1-42}$ in mice. Treatment with the ethanol extract of A. lappa L. roots improved working and reference memory in mice in the Y-maze and passive avoidance tests. To detect the lipid peroxidation levels, malondialdehyde was measured. To purify the bioactive compound, active fractions and components of A. lappa L. roots were isolated by partitioning, open column chromatography, TLC, high-performance liquid chromatography, electron ionization mass spectrometry, and $^1H/^{13}C$-nuclear magnetic resonance. We identified the bioactive compound as quinic acid, which is a powerful antioxidant agent. Ultimately, the roots of A. lappa L. may become available as an antioxidant food for Alzheimer's disease patients and those with other oxidative stress-induced disorders.

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