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논문 기본 정보

자료유형
학술저널
저자정보
Ahmadabad, Hasan Namdar (Department of Pathobiology and Medical Laboratory Science, School of Medicine, North Khorasan University of Medical Sciences) Jafari, Sabah Kayvan (Department of Biology, Payame Noor University of Mashhad) Firizi, Maryam Nezafat (Department of Pathobiology and Medical Laboratory Science, School of Medicine, North Khorasan University of Medical Sciences) Abbaspour, Ali Reza (Department of Biotechnology and Molecular Sciences, School of Medicine, North Khorasan University of Medical Sciences) Gharib, Fahime Ghafoori (Department of Biotechnology and Molecular Sciences, School of Medicine, North Khorasan University of Medical Sciences) Ghobadi, Yusef (Department of Immunology, School of Medicine, Tarbiat Modares University) Gholizadeh, Samira (Department of Immunology, School of Medicine, Tarbiat Modares University)
저널정보
대한생식의학회 Clinical and experimental reproductive medicine : CERM Clinical and experimental reproductive medicine : CERM 제43권 제1호
발행연도
2016.1
수록면
15 - 25 (11page)

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Objective: In the present study, we aimed to evaluate the effects of high doses of dexamethasone (DEX) in early pregnancy on pregnancy outcomes. Methods: Pregnant BALB/c mice were treated with high-dose DEX in the experimental group or saline in the control group on gestational days (GDs) 0.5 to 4.5. Pregnant mice were sacrificed on GDs 7.5, 13.5, or 18.5 and their peripheral blood, placentas, fetuses, and uterine tissue were collected. Decidual and placenta cell supernatants were examined to evaluate the effect of DEX on the proliferation of mononuclear cells, the quantity of uterine macrophages and uterine natural killer (uNK) cells, and levels of progesterone and $17{\beta}-estradiol$, as determined by an 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay, immunohistochemistry, and enzyme-linked immunosorbent assay, respectively. We also were measured fetal and placental growth parameters on GD 18.5. Results: We found that high doses of DEX were associated with an increased abortion rate, enhancement of the immunosuppressive effect of the decidua, alterations in placental growth parameters, decreased progesterone and $17{\beta}-estradiol$ levels, and a reduced frequency of macrophages and uNK cells. Conclusion: Our data suggest that the high-dose administration of DEX during early pregnancy negatively affected pregnancy outcomes.

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