지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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To quantify the abundance of the harmful dinoflagellate Cochlodinium polykrikoides in natural seawaters, we developed the innovative procedure using a droplet digital PCR (ddPCR) with C. polykrikoides-specific primers targeting the internal transcription sequence (ITS). The abundance of C. polykrikoides was estimated by the specific copy number of target ITS DNA segments per cell in cultures and natural water samples. The copy number per C. polykrikoides cell as acquired by ddPCR was $157{\pm}16$, which was evaluated against known cell numbers through a simplified protocol preparing DNAs. The abundances of C. polykrikoides in the waters of different locations estimated by ddPCR agreed with the number of cells visually counted under a microscope. This protocol was used to measure the abundance of C. polykrikoides close to and further off the southern coast of Korea in August of 2016 and 2017. The practical application showed that this method can reduce time for analysis and increase accuracy.
목차
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참고문헌 (30)
참고문헌 신청
Bereiter-Hahn J / 1990 / Behavior of mitochondria in the living cell / Int Rev Cytol 122:1~63
Buchheim, M. A / 2011 / Internal transcribed spacer 2 (nu ITS2 rRNA) sequence-structure phylogenetics: towards an automated reconstruction of the green algal tree of life / PLoS One 6:e16931
Cho, E. S / 2001 / A comparative study of the harmful dinoflagellates Cochlodinium polykrikoides and Gyrodinium impudicum using transmission electron microscopy, fatty acid composition, carotenoid content, DNA quantification and gene sequences / Bot. Mar 44:57~66
Coleman, A. W / 1997 / Ribosomal DNA ITS-1 and ITS-2 sequence comparisons as a tool for predicting genetic relatedness / J. Mol. Evol 45:168~177
Coyne, K. J / 2005 / Improved quantitative real‐time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard / Limnol. Oceanogr. Methods 3:381~391
Buchheim, M. A / 2011 / Internal transcribed spacer 2 (nu ITS2 rRNA) sequence-structure phylogenetics: towards an automated reconstruction of the green algal tree of life / PLoS One 6:e16931
Cho, E. S / 2001 / A comparative study of the harmful dinoflagellates Cochlodinium polykrikoides and Gyrodinium impudicum using transmission electron microscopy, fatty acid composition, carotenoid content, DNA quantification and gene sequences / Bot. Mar 44:57~66
Coleman, A. W / 1997 / Ribosomal DNA ITS-1 and ITS-2 sequence comparisons as a tool for predicting genetic relatedness / J. Mol. Evol 45:168~177
Coyne, K. J / 2005 / Improved quantitative real‐time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard / Limnol. Oceanogr. Methods 3:381~391
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