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논문 기본 정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제3호
- 발행연도
- 2021.1
- 수록면
- 358 - 367 (10page)
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초록· 키워드
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The World Health Organization (WHO) has declared the coronavirus disease 2019 (COVID-19) as an international health emergency. Current diagnostic tests are based on the reverse transcriptionquantitative polymerase chain reaction (RT-qPCR) method, which is the gold standard test that involves the amplification of viral RNA. However, the RT-qPCR assay has limitations in terms of sensitivity and quantification. In this study, we tested both qPCR and droplet digital PCR (ddPCR) to detect low amounts of viral RNA. The cycle threshold (CT) of the viral RNA by RT-PCR significantly varied according to the sequences of the primer and probe sets with in vitro transcript (IVT) RNA or viral RNA as templates, whereas the copy number of the viral RNA by ddPCR was effectively quantified with IVT RNA, cultured viral RNA, and RNA from clinical samples. Furthermore, the clinical samples were assayed via both methods, and the sensitivity of the ddPCR was determined to be equal to or more than that of the RT-qPCR. However, the ddPCR assay is more suitable for determining the copy number of reference materials. These findings suggest that the qPCR assay with the ddPCR defined reference materials could be used as a highly sensitive and compatible diagnostic method for viral RNA detection.
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참고문헌 (61)
참고문헌 신청
WHO / 2015 / Summary of probable SARS cases with onset of illness from 1 November 2002 to 31 July 2003 / WHO
Fung TS / 2019 / Human coronavirus: host-pathogen interaction / Annu. Rev. Microbiol 73:529~557
Li W / 2006 / Animal origins of the severe acute respiratory syndrome coronavirus : insight from ACE2-S-protein interactions / J. Virol 80:4211~4219
Wu K / 2012 / Mechanisms of host receptor adaptation by severe acute respiratory syndrome coronavirus / J. Biol. Chem 287:8904~8911
Ge XY / 2013 / Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor / Nature 503:535~538
Fung TS / 2019 / Human coronavirus: host-pathogen interaction / Annu. Rev. Microbiol 73:529~557
Li W / 2006 / Animal origins of the severe acute respiratory syndrome coronavirus : insight from ACE2-S-protein interactions / J. Virol 80:4211~4219
Wu K / 2012 / Mechanisms of host receptor adaptation by severe acute respiratory syndrome coronavirus / J. Biol. Chem 287:8904~8911
Ge XY / 2013 / Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor / Nature 503:535~538
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