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자료유형
학술저널
저자정보
Park, So-Yun (South Sea Environment Research Department, Korea Ocean Research and Development Institute) Kim, So-Jung (Kyeongbuk Institute for Marine Bio-Industry) Rhee, Yong (Department of Genetic Engineering, SungKyunKwan University) Yum, Seung-Shic (South Sea Environment Research Department, Korea Ocean Research and Development Institute) Kwon, Tae-Dong (Department of Entomology, Ohio State University) Lee, Taek-Kyun (South Sea Environment Research Department, Korea Ocean Research and Development Institute)
저널정보
대한독성유전단백체학회 Molecular & cellular toxicology Molecular & cellular toxicology 제5권 제3호
발행연도
2009.1
수록면
265 - 271 (7page)

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Concentrations of industrial, agricultural and natural chemicals have been increasing in secondary effluents without their combined sub-lethal effects having been elucidated. In this study, two assays (the comet and acetylcholinesterase assays) were combined to evaluate the genotoxic and neurotoxic effects of effluent from the Noksan wastewater treatment plant (WWTP) on two local marine fish species (flounder and sea eel). The fish were exposed to WWTP secondary effluent that had been diluted with filtered seawater to final concentrations of 1%, 10% and 50%. Analysis of fish samples collected 3 and 5 days after exposure showed that DNA damage occurred in flounder exposed to 50% effluent and in sea eels exposed to 10% or 50% effluent. Furthermore, it was found that acetylcholinesterase (EC:3.1.1.7, AChE) activity decreased in both species when exposed to 10% effluent, indicating the presence of large amounts of genotoxic and neurotoxic chemicals in the effluent. Our results indicate that the comet and AChE assays are promising tools for biomonitoring of secondary effluents.

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