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자료유형
학술저널
저자정보
Indiragandhi, Pandiyan (Tamil Nadu Agricultural University) Yoon, Chang-Mann (Department of Agricultural Biology, College of Agriculture, Life and Environment Sciences, Chungbuk National University) Yang, Jeong-Oh (Department of Agricultural Biology, College of Agriculture, Life and Environment Sciences, Chungbuk National University) Cho, Soo-Won (Department of Agricultural Biology, College of Agriculture, Life and Environment Sciences, Chungbuk National University) Sa, Tong-Min (Department of Agricultural Chemistry, Chungbuk National University) Kim, Gil-Hah (Department of Agricultural Biology, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
저널정보
한국응용생명화학회 Journal of applied biological chemistry Journal of applied biological chemistry 제53권 제5호
발행연도
2010.1
수록면
605 - 617 (13page)

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Whiteflies are sap-sucking insects belonging to the hemipteran order. They are well known for their menace to agriculture, as pests and vectors, and are reported for their bacterial and Rickettsia association in B biotype. In the present investigation, culture-dependent and -independent methods were used to reveal the bacterial phylotypes associated with B and Q biotypes. Cultivable bacterial phylotypes varied with respect to growth media and biotypes. Twenty different bacterial genera, including 31 species belong to Actinobacteria, 'alpha'-, 'beta'-, 'gamma'- Proteobacteria, and Firmicutes were isolated from both the biotypes. Of the seventeen phylotypes, Bacillus, Kocuria, Moraxellla, Micrococcus, Sphingomonas and Staphylococcus were common to both B and Q biotypes. Moreover, B biotype was associated with Acinetobacter, Deinococcus, Modestobacter, Microbacterium, and Pseudomonas, whereas Q biotype was associated with Arthrobacter, Bradyrhizobium, Janibacter, Morganella, Naxibacter, and Streptomyces. Application of a culture-independent method revealed the presence of additional symbiotic bacteria: Rickettsia in B biotype and Halomonas in Q biotype, as well as primary endosymbiont in both biotypes, which could not be obtained through culture-dependent method. Presence of Staphylococcus, Micrococcus (in both B and Q biotypes), and Bacillus (only in B biotype) in all developmental stages of B. tabaci indicated their close association with host insect.

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