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논문 기본 정보

자료유형
학술저널
저자정보
Ha, Ki-Young (Daisy Plastic Surgery Clinic) Park, Hojin (Department of Plastic Surgery, Korea University Anam Hospital, Korea University College of Medicine) Park, Seung-Ha (Department of Plastic Surgery, Korea University Anam Hospital, Korea University College of Medicine) Lee, Byung-Il (Department of Plastic Surgery, Korea University Anam Hospital, Korea University College of Medicine) Ji, Yi-Hwa (Department of Plastic Surgery, Korea University Ansan Hospital, Korea University College of Medicine) Kim, Tae-Yeon (Department of Plastic Surgery, Bundang Jesaeng General Hospital) Yoon, Eul-Sik (Department of Plastic Surgery, Korea University Anam Hospital, Korea University College of Medicine)
저널정보
대한성형외과학회 Archives of plastic surgery : APS Archives of plastic surgery : APS 제42권 제6호
발행연도
2015.1
수록면
677 - 685 (9page)

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Background The survival rate of grafted fat is difficult to predict, and repeated procedures are frequently required. In this study, the effects of the freezing period of harvested adipose tissue and the addition of human adipose tissue-derived stem cells (ASCs) on the process of fat absorption were studied. Methods Adipose tissue was obtained from patients who underwent a lipoaspirated fat graft. The fat tissue was cryopreserved at $-20^{\circ}C$ in a domestic refrigerator. A total of 40 nude mice were used. The mice in the experimental group received three different subcutaneous injections in the back: an injection of fresh fat and ASCs, an injection of fat that had been frozen for one month and ASCs, and an injection of fat that had been frozen for two months and ASCs. The control mice received fat grafts without ASCs. The mice were sacrificed at four or eight weeks after the procedure, and the grafted fat tissues were harvested. The extracted fat was evaluated using photographic analysis, volume measurements, and histological examination. Results In the control group, the fat resorption rates four weeks after transplantation in the grafts of fresh fat, fat that had been frozen for one month, and fat that had been frozen for two months were 21.14%, 22.46%, and 42.56%, respectively. In the experimental group, the corresponding resorption rates were 6.68%, 13.0%, and 33.9%, respectively. Conclusions ASCs can increase the fat graft survival rate. The use of ASCs in fat grafting can reduce the need for repeated fat grafts and provide good long term results.
#Stem cells #Cryopreservation #Injections, subcutaneous #Adipose tissue

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참고문헌 (23)

참고문헌 신청
Rohrich RJ / 2004 / In search of improved fat transfer viability: a quantitative analysis of the role of centrifugation and harvest site / Plast Reconstr Surg 113:391~395 google schola Boschert MT / 2002 / Analysis of lipocyte viability after liposuction / Plast Reconstr Surg 109:761~765 google schola Coleman SR. / 1997 / Facial recontouring with lipostructure / Clin Plast Surg 24:347~367 google schola Pu LL / 2005 / The viability of fatty tissues within adipose aspirates after conventional liposuction: a comprehensive study / Ann Plast Surg 54:288~292 google schola Shiffman MA / 2001 / Fat transfer techniques: the effect of harvest and transfer methods on adipocyte viability and review of the literature / Dermatol Surg 27:819~826 google schola

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