Melatonin Protects Human Adipose-Derived Stem Cells from Oxidative Stress and Cell Death

Tan, Shaun S.; Han, Xiaolian; Sivakumaran, Priyadharshini; Lim, Shiang Y.; Morrison, Wayne A.

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자료유형: 학술저널

저자정보: Tan, Shaun S. (Bernard O'Brien Institute Department, St. Vincent's Institute of Medical Research) Han, Xiaolian (Bernard O'Brien Institute Department, St. Vincent's Institute of Medical Research) Sivakumaran, Priyadharshini (Bernard O'Brien Institute Department, St. Vincent's Institute of Medical Research) Lim, Shiang Y. (Bernard O'Brien Institute Department, St. Vincent's Institute of Medical Research) Morrison, Wayne A. (Bernard O'Brien Institute Department, St. Vincent's Institute of Medical Research)

저널정보: 대한성형외과학회 Archives of plastic surgery : APS Archives of plastic surgery : APS 제43권 제3호

발행연도: 2016.1

수록면: 237 - 241 (5page)

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Background Adipose-derived stem cells (ASCs) have applications in regenerative medicine based on their therapeutic potential to repair and regenerate diseased and damaged tissue. They are commonly subject to oxidative stress during harvest and transplantation, which has detrimental effects on their subsequent viability. By functioning as an antioxidant against free radicals, melatonin may exert cytoprotective effects on ASCs. Methods We cultured human ASCs in the presence of varying dosages of hydrogen peroxide and/or melatonin for a period of 3 hours. Cell viability and apoptosis were determined with propidium iodide and Hoechst 33342 staining under fluorescence microscopy. Results Hydrogen peroxide (1-2.5 mM) treatment resulted in an incremental increase in cell death. 2 mM hydrogen peroxide was thereafter selected as the dose for co-treatment with melatonin. Melatonin alone had no adverse effects on ASCs. Co-treatment of ASCs with melatonin in the presence of hydrogen peroxide protected ASCs from cell death in a dose-dependent manner, and afforded maximal protection at $100{\mu}M$ (n=4, one-way analysis of variance P<0.001). Melatonin co-treated ASCs displayed significantly fewer apoptotic cells, as demonstrated by condensed and fragmented nuclei under fluorescence microscopy. Conclusions Melatonin possesses cytoprotective properties against oxidative stress in human ASCs and might be a useful adjunct in fat grafting and cell-assisted lipotransfer.

#Melatonin #Hydrogen peroxide #Mesenchymal stem cell #Cytoprotection

참고문헌 (20)

참고문헌 신청

Fraser JK / 2006 / Fat tissue: an underappreciated source of stem cells for biotechnology / Trends Biotechnol 24:150~154

Li CY / 2015 / Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy / Stem Cell Res Ther 6:55

Monsel A / 2014 / Cell-based therapy for acute organ injury: preclinical evidence and ongoing clinical trials using mesenchymal stem cells / Anesthesiology 121:1099~1121

Luchetti F / 2010 / Melatonin signaling and cell protection function / FASEB J 24:3603~3624

Sanchez-Barcelo EJ / 2010 / Clinical uses of melatonin: evaluation of human trials / Curr Med Chem 17:2070~2095

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