지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 402 - 409 (8page)
이용수
초록· 키워드
오류제보하기
A novel peptide having free radical scavenging activity was separated, using an on-line high-performance liquid chromatography (HPLC) - ABTS screening method, from bovine skim milk fermented by Lactococcus lactis SL6 (KCTC 11865BP). It was further purified using reverse phase-HPLC (RP-HPLC) and sequenced by RP-HPLC-tandem mass spectrometry. The amino acid sequence of the identified peptide was determined to be Phe-Ser-Asp-Ile-Pro-Asn-Pro-Ile-Gly-Ser-Glu-Asn-Ser-Glu-Lys-Thr-Thr-Met-Pro-Leu-Trp (2,362 Da), which is corresponding to the C-terminal fragment of bovine ${\alpha}_{s1}$-casein (f179-199). The hydroxyl radicals scavenging activity ($IC_{50}$ $28.25{\pm}0.96{\mu}M$) of the peptide chemically synthesized based on the MS/MS data showed a slightly lower than that of the natural antioxidant Trolox ($IC_{50}$ $15.37{\pm}0.52{\mu}M$). Furthermore, derivatives of the antioxidant peptide were synthesized. The antioxidative activity of the derivatives whose all three proline residues replaced by alanine significantly decreased, whereas replacement of two proline residues in N-terminal region did not affect its antioxidative activity, indicating that $3^{rd}$ proline in C-terminal region is critical for the antioxidative activity of the peptide identified in this study. In addition, N-terminal region of the antioxidant peptide did not show its activity, whereas C-terminal region maintained antioxidative activity, suggesting that C-terminal region of the peptide is important for antioxidative activity.
목차
등록된 정보가 없습니다.
참고문헌 (34)
참고문헌 신청
Chang, O. K. / 2013 / Casein hydrolysis by Bifidobacterium longum KACC91563 and antioxidant activities of peptides derived therefrom / J. Dairy Sci 96:5544~5555
Chen, H. -M. / 1998 / Antioxidative properties of histidinecontaining peptides designed from peptide fragments found in the digests of a soybean protein / J. Agric. Food Chem 46:49~53
Cheung, H. S. / 1980 / Binding of peptide substrates and inhibitors of angiotensin-converting enzyme. Importance of the COOH-terminal dipeptide sequence / J. Biol. Chem 255:401~407
Christensen, J. E. / 1999 / Peptidases and amino acid catabolism in lactic acid bacteria / Antonie Leeuwenhoek 76:217~246
Clare, D. A. / 2003 / Biodefense properties of milk: The role of antimicrobial proteins and peptides / Curr. Pharm. Des 9:1239~1255
Chen, H. -M. / 1998 / Antioxidative properties of histidinecontaining peptides designed from peptide fragments found in the digests of a soybean protein / J. Agric. Food Chem 46:49~53
Cheung, H. S. / 1980 / Binding of peptide substrates and inhibitors of angiotensin-converting enzyme. Importance of the COOH-terminal dipeptide sequence / J. Biol. Chem 255:401~407
Christensen, J. E. / 1999 / Peptidases and amino acid catabolism in lactic acid bacteria / Antonie Leeuwenhoek 76:217~246
Clare, D. A. / 2003 / Biodefense properties of milk: The role of antimicrobial proteins and peptides / Curr. Pharm. Des 9:1239~1255
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Antioxidant activity of a novel antioxidative peptide isolated from a marine Chlorella ellipsoidea
한국수산과학회 양식분과 학술대회
2018 .05
-
Comparison of Antioxidant Activities of Hydrolysates of Domestic and Imported Skim Milk Powders Treated with Papain
한국축산식품학회지
2015 .01
-
Bioactive Peptides in Milk and Dairy Products: A Review
한국축산식품학회지
2015 .01
-
Fermentation of Vegetable Juice by Lactococcus lactis and Its Antimicrobial Activity
한국식품영양과학회 학술대회발표집
2017 .10
-
Characterization of antioxidative peptide purified from black eelpout (Lycodes diapterus) hydrolysate
Fisheries and Aquatic Sciences
2019 .01
-
Novel Antioxidant Peptides Derived from Brown Rice Fermented by Limosilactobacillus reuteri and Their Cytoprotective effects on In-vitro Radical Assays and Ex-vivo H₂O₂-induced Oxidative Stress
한국식품영양과학회 학술대회발표집
2021 .10
-
Production of Dipeptidyl Peptidase-IV Inhibitory Peptides from Germinated Jack Bean [Canavalia ensiformis (L.) DC.] Flour
Preventive Nutrition and Food Science
2023 .06
-
Comparison of analysis methods for human blood peptides
한국식품영양과학회 학술대회발표집
2022 .10
-
Isolation of antioxidant peptide from sandfish (Arctoscopus japonicus) roe hydrolysate
Food Science and Preservation
2017 .07
-
Amelioration of Muscle Wasting by C-Peptide in Glucocorticoid-Induced Skeletal Muscle Atrophy
한국식품영양과학회 학술대회발표집
2021 .10
-
Correlation analysis between blood peptides and clinical data
한국식품영양과학회 학술대회발표집
2024 .10
-
적미 미강 추출 분획물의 항산화 활성
한국식품영양과학회지
2019 .01
-
Anti-proliferative Effect of a Novel Anti-oxidative Peptide in Hanwoo Beef on Human Colorectal Carcinoma Cells
한국축산식품학회지
2018 .01
-
Peptides Derived from Insect Enhanced Hanwoo Satellite Cell Proliferation
한국식품영양과학회 학술대회발표집
2021 .10
-
Antioxidant and angiotensin-I converting enzyme inhibitory peptides from Hippocampus abdominalis
한국수산과학회 양식분과 학술대회
2021 .04
-
β-glucano oligosaccharide as selective substrates for Lactococcus lactis subsp. lactis
한국수산과학회 양식분과 학술대회
2016 .10
-
Biological activity of peptides purified from fish skin hydrolysates
Fisheries and Aquatic Sciences
2019 .01
-
Comparative study on the bioavailability of peptide extracts from Jeju black pigs and three-way crossbred pigs
한국축산학회지
2024 .09
-
Study on disease biomarker development through blood peptide analysis
한국식품영양과학회 학술대회발표집
2024 .10
-
Preparation of house cricket peptide by enzymatic hydrolysis and development of herbal peptide drink
Food Science and Preservation
2024 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0