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Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids
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논문 기본 정보

Type
Academic journal
Author
Mulyani, Sri (Department of Food and Agricultural Product Technology, Gadjah Mada University) Setyabudi, Francis.M.C. Sigit (Department of Food and Agricultural Product Technology, Gadjah Mada University) Pranoto, Yudi (Department of Food and Agricultural Product Technology, Gadjah Mada University)
Journal
한국축산식품학회 한국축산식품학회지 한국축산식품학회지 제37권 제5호 KCI Accredited Journals
Published
2017.1
Pages
708 - 715 (8page)

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Physicochemical Properties of Gelatin Extracted from Buffalo Hide Pretreated with Different Acids
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The acid pretreatment of collagen molecules disrupts their crosslinks and assists in the release of acid-soluble proteins, fats, and other components. Generally, to achieve optimum extraction efficiency, strong acids may be used at a lower acid concentration compared to weak acids. This study aimed to determine the yield and physicochemical properties of gelatins extracted from buffalo hides pretreated with different acids. Hides were extracted with hydrochloric, citric, and acetic acids at concentrations of 0.3, 0.6, 0.9, 1.2, and 1.5 M. A completely randomized design and the least significant difference test were used in the experimental design, and all measurements were performed in triplicate. The highest yield (29.17%) was obtained from pretreatment with 0.9 M HCl. The gel strength did not differ significantly (p>0.05) according to acid type (280.26-259.62 g Bloom), and the highest viscosity was obtained from the 0.6 M citric acid pretreatment. All the gelatins contained ${\alpha}$- and ${\beta}$-chain components and several degraded peptides (24-66 kDa). The color and Fourier-transform infrared spectrum of the gelatin extracted using 0.9 M HCl were similar to those of commercial bovine skin gelatin. In general, the physicochemical properties of the gelatin complied with the industry standard set by the Gelatin Manufacturers Institute of America, revealing that buffalo hide could serve as a potential alternative source of gelatin.
#acid pretreatment #buffalo hide #gelatin #physicochemical properties #yield

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References (30)

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Ahmad, M / 2011 / Characteristics of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as influenced by acid pretreatment and extraction time / Food Hydrocoll 25:281~288 google schola AOAC / 2005 / Official Methods of Analyses Association (18th Ed.) / Association of Analytical Chemist google schola Benjakul, S. / 2009 / Characteristic of gelatin from the skins of bigeye snapper, Priacanthus tayenus and Priachantus macracanthus / Food Chem 116:445~451 google schola Giménez, B. / 2005 / Use of lactic acid for extraction of fish skin gelatin / Food Hydrocoll 19:941~950 google schola GMIA / Gelatin handbook google schola

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