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논문 기본 정보

자료유형
학술저널
저자정보
Park, Sang Hyoun (Animal Biotechnology Division, National Institute of Animal Science, RDA) Oh, Keon Bong (Animal Biotechnology Division, National Institute of Animal Science, RDA) Ock, Sun-A (Animal Biotechnology Division, National Institute of Animal Science, RDA) Byun, Sung June (Animal Biotechnology Division, National Institute of Animal Science, RDA) Lee, Hwi-Cheul (Animal Biotechnology Division, National Institute of Animal Science, RDA) Kumar, Suresh (Animal Biotechnology Division, National Institute of Animal Science, RDA) Lee, Sung Gu (Unit of Polar Genomics, Korea Polar Research Institute, KIOST) Woo, Jae-Seok (Animal Biotechnology Division, National Institute of Animal Science, RDA)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제33권 제3호
발행연도
2018.1
수록면
185 - 194 (10page)

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The aim of this study was performed to evaluate the effects of ice-binding protein from the arctic yeast Leucosporidium (LeIBP) supplementation on cryopreservation of boar sperm. The collected semen was diluted ($1.5{\times}10^8/ml$) in lactose egg yolk (LEY) and cooled at $5^{\circ}C$ for 3 h. The cooled semen was then diluted ($1{\times}10^8/ml$) in LeIBP containing LEY with 9% glycerol and maintained at $5^{\circ}C$ for 30 min. The semen was divided into six experimental groups (control, 0.001, 0.005, 0.01, 0.05 and 0.1 mg/ml of LeIBP). The straws were kept on above the liquid nitrogen ($LN_2$) vapors for 20 minutes and then plunged into $LN_2$. After thawing, computer-assisted sperm analysis was used for sperm motility and flow cytometry was performed to assess the viability, acrosome integrity (FITC-PSA/PI), ROS (DCF/PI), lipid peroxidation (BODIPY C11/PI) and apoptosis (Annexin V/PI), respectively. No significant responses were observed for sperm motility. However, sperm viability was significantly increased on 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). In addition, acrosome integrity was significantly increases LeIBP groups (P < 0.05) and both ROS and lipid peroxidation level were lower in all LeIBP groups than those of control (P < 0.05). On the other hand, a significant higher apoptosis rate was observed in 0.05 and 0.1 mg/ml of LeIBP groups compared to control (P < 0.05). It can be assumed that a supplementation of LeIBP in boar sperm freezing extender is an effective method to increase the sperm qualities after cryopreservation.

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