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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Hyun-Jong (Animal Genetic Resources Station, NLAI, RDA) Cho, Sang-Rae (Animal Genetic Resources Station, NLAI, RDA) Choe, Chang-Yong (Animal Genetic Resources Station, NLAI, RDA) Choi, Sun-Ho (Animal Genetic Resources Station, NLAI, RDA) Son, Dong-Soo (Animal Genetic Resources Station, NLAI, RDA) Kim, Sung-Jae (Animal Genetic Resources Station, NLAI, RDA) Sang, Byung-Don (Animal Genetic Resources Station, NLAI, RDA) Han, Man-Hye (Animal Genetic Resources Station, NLAI, RDA) Ryu, Il-Sun (Animal Genetic Resources Station, NLAI, RDA) Kim, In-Cheul (Animal Genetic Resources Station, NLAI, RDA) Kim, Il-Hwa (Department of Veterinary Medicine, Chungbuk National University) Lee, Woon-Kyu (Department of Laboratory Animal Medicine, Yonsei University) Im, Kyung-Soon (Department of Animal Biotechnology, Seoul National University)
저널정보
한국동물번식학회 한국수정란이식학회지 한국수정란이식학회지 제22권 제4호
발행연도
2007.1
수록면
245 - 249 (5page)

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초록· 키워드

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The aim of present experiment was to examine hatching rate as in vitro indicator of viability of porcine embryos before early stage embryo transfer such as zygotes or 2-cell stage embryos. Cumulus-oocyte complexes (COCs) collected from ovaries were matured in North Carolina State University 23 (NCSU-23) containing 10% porcine follicular fluid (pFF), 10 ng/ml epidermal growth factor (EGF), $10{\mu}g/ml$ follicle stimulating hormone (FSH), $35{\mu}g/ml$ luteinizing hormone (LH), and 1mg/ml cysteine. After 24 hours, the COCs were transferred to the same medium without hormones. After 65h of maturation, oocytes were exposed to phosphate buffered saline (PBS) with 7% ethanol (v/v) for 7 minutes, and then the oocytes were washed and cultured in tissue culture medium (TCM) 199 containing 5 ug/ml cytochalasin B for 5h at $38.5^{\circ}C$ in an atmosphere of 5% $CO_2$ and 95% air with high humidity. After cytochalasin B treatment, the presumptive parthenotes were cultured in porcine zygote medium (PZM)-5 and cleavage of the parthenotes was assessed at 72h of activation, Normally cleaved parthenotes were cultured for an additional 8 days to evaluate their ability to develop to blastocyst and hatching stages. The fetal bovine serum (FBS) were added at Day 4 or 5 with concentrations of 2.5, 5 or 10%. The blastocyst rates were ranged within $39.1{\sim}70%$ in each treatment. However hatching rate was dramatically decreased in non-addition group. In this experiment, embryo viability in female reproductive tract may be estimated before embryo transfer with in vitro culture adding FBS by hatching ability.

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