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논문 기본 정보

자료유형
학술저널
저자정보
Moon, Hyo-Jin (Division of Animal Biotechnology, National Institute of Animal Science, RDA) Shim, Joo-Hyun (Division of Animal Biotechnology, National Institute of Animal Science, RDA) Hwang, In-Sun (Division of Animal Biotechnology, National Institute of Animal Science, RDA) Park, Mi-Rung (Division of Animal Biotechnology, National Institute of Animal Science, RDA) Kim, Dong-Hoon (Division of Animal Biotechnology, National Institute of Animal Science, RDA) Ko, Yeoung-Gyu (Division of Animal Biotechnology, National Institute of Animal Science, RDA) Park, Choon-Keun (College of Animal Science, Kangwon National University) Im, Gi-Sun (Division of Animal Biotechnology, National Institute of Animal Science, RDA)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제33권 제2호
발행연도
2009.1
수록면
85 - 91 (7page)

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In this study, in vitro maturation system using fetal bovine serum (FBS) or porcine follicular fluid (pFF) was investigated to produce comparable oocytes to those derived from in vivo. Control group of oocytes was cultured in TCM 199 supplemented with 0.1% polyvinyl alcohol (PVA). Other three groups of oocytes were cultured in TCM 199 supplemented with 10% FBS, 10% pFF or 5% FBS + 5% pFF, respectively. After 44 h maturation, oocytes with the first polar body were activated with two electric pulses (DC) of 1.2 kv/cm for 30 ${\mu}sec$. Also, matured oocytes of four groups were reconstructed and fused. Reconstructed embryos were cultured in PZM-3 under 5% $CO_2$ in air at $38.5^{\circ}C$ for 6 days. The oocytes matured in the medium supplemented with FBS or/and pFF showed significantly higher maturation rates (64.0 vs. 73.9 to 85.2%). In PA embryos, cleavage rates (89.7 vs. 77.1 to 86.6%) and blastocysts rates (30.0 vs. 16.2 to 26.2%) were significantly higher in pFF group (p<0.05). In NT embryos, there was no difference among treatments in cleavage rate, but the blastocyst rates (28.5 vs. 15.5 to 24.6%) were significantly higher in pFF group (p<0.05). The apoptosis rate was significantly higher (p<0.05) in the control than other groups (10.8 vs. 4.9 to 8.2% for PA, 3.1 vs. 0.5 to 1.3% for NT). In order to select the comparable oocyte to in vivo oocytes, each group of oocytes was stained with Brilliant cresyl blue (BCB) after 42h maturation. The matured oocytes were separated according to color of cytoplasm; stained group (BCB+) and unstained group (BCB-). The oocytes matured in the presence of FBS or/and pFF showed significantly higher staining rates (70.3 to 72.7 vs. 35.1%) (p<0.05). To verify the fact that the supplementation of FBS or/and pFF can increase the maturation rates, cdc2 kinase activity, the catalytic subunit of MPF, was determined. The cdc2 kinase activity of the oocytes matured in the medium supplemented with FBS or/and pFF was significantly higher than control group (6.7 to 9.3 vs. 3.8). In conclusion, the supplementation of FBS or/and pFF can support in vitro maturation rate of porcine oocytes through the increment of cdc2 kinase activity level in the cytoplasm.
#FBS #pFF #Porcine embryos #BCB #Cdc2 kinase

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