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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Jong-Mu (National Research Laboratory of Molecular Embryology, Department of Animal Sciences, Chungbuk National University) Ko, Yeoung-Gyu (Animal Biotechnology Division, National Livestock Research institute) Seong, Hwan-Hoo (Animal Biotechnology Division, National Livestock Research institute) Chung, Hak-Jae (Animal Biotechnology Division, National Livestock Research institute) Chang, Won-Kyong (Animal Biotechnology Division, National Livestock Research institute) Kim, Nam-Hyung (National Research Laboratory of Molecular Embryology, Department of Animal Sciences, Chungbuk National University)
저널정보
한국동물번식학회 한국동물번식학회지 한국동물번식학회지 제31권 제1호
발행연도
2007.1
수록면
21 - 28 (8page)

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During early embryo development, Oct-4 is an important transcription factor for the early differentiation the present study was first examined methylation status in distal enhancer and promoter region of Oct-4 during mouse pre-implantation embryo development. In oocyte and sperm, high methylation was observed in both distal and proximal of promoter in Oct-4. Following fertilization relatively high methylation level remained until 8-cell stage embryos, but decreased at the morula and blastocyst stage. Specific gene knock down of Oct-4 by siRNA injection into zygote induced higher methylation rates of both distal and proximal region of promoter of Oct-4. These results suggest a functional link between the DNA methylation status of distal and promoter resign in the Oct-4 gene and the gene sequence-specific transcriptional silencing by exogenous siRNA injection during mouse preimplantation embryos.

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