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자료유형
학술저널
저자정보
Yeo, S.E. (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology[KRIBB], Department of Biology, Chungnam National University) Kang, Y.K. (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology[KRIBB]) Koo, D.B. (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology[KRIBB]) Han, J.S. (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology[KRIBB]) Yu, K. (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology[KRIBB]) Kim, C.H. (Department of Biology, Chungnam National University) Park, H. (Department of Biotechnology, Taegu University) Chang, W.K. (National Livestock Research Institute) Lee, K.K. (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology[KRIBB]) Han, Y.M. (Laboratory of Development and Differentiation, Korea Research Institute of Bioscience and Biotechnology[KRIBB])
저널정보
한국동물번식학회 한국가축번식학회지 한국가축번식학회지 제27권 제4호
발행연도
2003.1
수록면
309 - 315 (7page)

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DNA methylation at CpG sites, which is a epigenetic modification, is associated with gene expression without change of DNA sequences. During early mouse embryogenesis, dynamic changes of DNA methylation occur. In this study, DNA methylation patterns of porcine embryos produced in vivo and in vitro were examined at various developmental stages by the immunocytochemical staining method. Interestingly, active demethylation was not observed on the paternal pronucleus of porcine zygotes. However, differences were detected in the passive demethylation process between in vivo and in vitro embryos. There was no change in the DNA methylation state until the blastocyst stage of in vivo embryos, whereas partial demethylation was observed in several blastomeres from a 4 cell stage to a morula stage of in vitro embryos. The whole genome of inner cell mass (ICM) and trophectoderm (TE) cells in porcine blastocysts were evenly methylated without de novo methylation. Our findings demonstrate that genome-wide demethylation does not occur in pig embryos during preimplantation development unlike murine and bovine embryos. It indicates that the machinery regulating epigenetic reprogramming may be different between species.

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