지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.9
- 수록면
- 273 - 279 (7page)
- DOI
- 10.7841/ksbbj.2020.35.3.273
이용수
초록· 키워드
오류제보하기
Global warming is a major issue of modern climate change, and caused by the greenhouse effect mainly due to carbon dioxide emitted by burning fossil fuels. To reduce the emission of carbon dioxide, scientists have focused on replacing fossil fuels with renewable energy. Biofuel, one form of renewable energy derived from the carbon sources of natural biomass, can contribute to reduced carbon dioxide emission. In this study, Escherichia coli was engineered for the production of fatty acid ethyl ester (FAEE), biodiesel derived from fatty acids. For production of FAEE, pyruvate decarboxylase (pdc), alcohol dehydrogenase II (adhB), acyl-Coenzyme A : diacylglycerol acyltransferase (atfA), and fatty acid synthesis (FAS) pathway genes were selected and cloned on to various plasmids. We designed and introduced various combinations of expression constructs in E. coli to enhance FAEE production. As a result, a 3.88-fold increase in FAEE production was achieved by modifying the expression levels of the genes involved in the initiation step and the β-oxidation step. The production of FAEE at different growth phases as well as the effect of the culture conditions such as temperature and carbon sources on FAEE productivity were studied. As a future study, scale-up studies through fermenter cultivation would contribute to mass production of biodiesel.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (31)
참고문헌 신청
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Ramanathan, V. / 1988 / The greenhouse theory of climate change : a test by an inadvertent global experiment / Science 240:293~299
Nejat, P. / 2015 / A global review of energy consumption, CO2 emissions and policy in the residential sector(with an overview of the top ten CO2 emitting countries) / Renewable Sustainable Energy Rev 43:843~862
Cazenave, A. / 2014 / Sea level rise and its coastal impacts / Earth's Future 2:15~34
Merkx-Jacques, A. / 2018 / Engineering xylose metabolism in thraustochytrid T18 / Biotechnol. Biofuels 11:248
Ramanathan, V. / 1988 / The greenhouse theory of climate change : a test by an inadvertent global experiment / Science 240:293~299
Nejat, P. / 2015 / A global review of energy consumption, CO2 emissions and policy in the residential sector(with an overview of the top ten CO2 emitting countries) / Renewable Sustainable Energy Rev 43:843~862
Cazenave, A. / 2014 / Sea level rise and its coastal impacts / Earth's Future 2:15~34
Merkx-Jacques, A. / 2018 / Engineering xylose metabolism in thraustochytrid T18 / Biotechnol. Biofuels 11:248
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UCI(KEPA) : I410-ECN-0101-2020-470-001290311