지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objectives: The aim of this study is to investigate the mechanisms involved in the anti-inflammatory and anti-tumor effects of Rhus verniciflua Stokes (RVS) on PMA-differentiated human monocytic leukemia THP-1 cells.
Methods: Cells were treated with various concentrations of RVS decoction (0-300μg/ml) for 24, 48, and 72h. Cell viability was evaluated by MTS/PMS assay. The expressions of MMP-2, MMP-9, TIMP-1, TIMP-2, iNOS and COX-2 mRNA and proteins were measured using RT-PCR and western blotting, respectively.
Results: RVS suppressed expression of MMP-2 and MMP-9 mRNA. It also down-regulated iNOS and COX-2 mRNA and protein expression. RVS inhibited NF-κB p65 activity and the phosphorylation of Akt and MAPK (ERK and p38 MAPK). Instead, the phosphorylation of JNK is increased at a very low concentration but decreased at higher concentrations.
Conclusion: RVS is regarded to inhibit the expression of MMP and TIMP as well as iNOS and COX-2 gene expression via directly inhibiting the activation of NF-κB and phosphorylation of MAPK pathway in THP-1 cells. This suggests RVS have potential to be used as a therapeutic agent for acute myeloid leukemia (AML).
Methods: Cells were treated with various concentrations of RVS decoction (0-300μg/ml) for 24, 48, and 72h. Cell viability was evaluated by MTS/PMS assay. The expressions of MMP-2, MMP-9, TIMP-1, TIMP-2, iNOS and COX-2 mRNA and proteins were measured using RT-PCR and western blotting, respectively.
Results: RVS suppressed expression of MMP-2 and MMP-9 mRNA. It also down-regulated iNOS and COX-2 mRNA and protein expression. RVS inhibited NF-κB p65 activity and the phosphorylation of Akt and MAPK (ERK and p38 MAPK). Instead, the phosphorylation of JNK is increased at a very low concentration but decreased at higher concentrations.
Conclusion: RVS is regarded to inhibit the expression of MMP and TIMP as well as iNOS and COX-2 gene expression via directly inhibiting the activation of NF-κB and phosphorylation of MAPK pathway in THP-1 cells. This suggests RVS have potential to be used as a therapeutic agent for acute myeloid leukemia (AML).
목차
Introduction
Materials and methods
Results
Discussion
References
참고문헌 (39)
참고문헌 신청
Döhner H / 2015 / Acute Myeloid Leukemia / N Engl J Med 373:1136~1152
Siegel R / 2013 / Cancer statistics, 2013 / CA Cancer J Clin 63(1):11~30
American Cancer Society / Cancer Facts and Figures 2017
Eun-Hye Park / 2015 / Nationwide statistical analysis of myeloid malignancies in Korea: incidence and survival rate from 1999 to 2012 / Blood Research 50(4):204~217
Yilmaz M / 2019 / Late relapse in acute myeloid leukemia (AML): clonal evolution or therapy-related leukemia? / Blood Cancer Journal 9(2):7
Siegel R / 2013 / Cancer statistics, 2013 / CA Cancer J Clin 63(1):11~30
American Cancer Society / Cancer Facts and Figures 2017
Eun-Hye Park / 2015 / Nationwide statistical analysis of myeloid malignancies in Korea: incidence and survival rate from 1999 to 2012 / Blood Research 50(4):204~217
Yilmaz M / 2019 / Late relapse in acute myeloid leukemia (AML): clonal evolution or therapy-related leukemia? / Blood Cancer Journal 9(2):7
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UCI(KEPA) : I410-ECN-0101-2021-519-000053683