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Effect of Fermented Soybean on the Proliferation and Growth in HaCaT and Fibroblast Cell
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대두 발효물이 인간 유래 피부세포의 증식 및 성장에 미치는 영향

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Type
Academic journal
Author
Eun-Joo Kim (경기대학교) Myung-Ryun Han (혜전대학교) So-Young Lee (경기대학교) Ae-Jung Kim (경기대학교)
Journal
Korea Academy Industrial Cooperation Society Journal of the Korea Academia-Industrial cooperation Society Vol.22 No.2 KCI Accredited Journals
Published
2021.2
Pages
326 - 335 (10page)

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Effect of Fermented Soybean on the Proliferation and Growth in HaCaT and Fibroblast Cell
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This study was undertaken to determine the effect of fermented soybean extract and its fractions on skin cell proliferation and growth. The extract was procured by the pepsin and Lactobacillus rhamnosus fermentation of soybean. LC-MS analysis was performed subsequent to soybean fermentation, and cell viability was measured by the WST-1 assay. Cell proliferation was observed to increase after exposing cells to the fermented soybean extract and its fractions at all concentrations tested (0~2,000 μg/mL). In particular, compared to the normal control group and 120 % proliferation of the EGF (epidermal growth factor) positive control group, 160~180 % cell proliferation was achieved at 800 μg/ml, indicating the excellent potential as an application material for skin aging inhibition and skin cell regeneration. In addition, we also examined the effects of fermented soybean extract and its fractions on wound healing ability, in HaCaT cells and fibroblasts. Our results indicate excellent cell migration abilities after treatment with fermented soybean extract and its fractions, as compared to the control treatment. Similar cell migration abilities were observed in the positive control group (EGF). Taken together, our results indicate that fermented soybean extract and its fractions (F4 and F5) exert amelioratory effects as a natural material for skin.
#Fermented Soybean Extract #Keratinocyte #Fibroblast #Proliferation #Migration

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D. Agyei, “Bioactive proteins and peptides from soybeans”, Recent Patents on Food, Nutrition & Agriculture , Vol.7, No.2, pp.100–107, Agu. 2015. DOI: https://doi.org/10.2174/221279840766615062913414 google schola S. P. Hong, U. H. Choi, S. G. An, “Skin barrier and antimicrobial activity”, The Journal of Skin Barrier Research , Vol.11, No.1, pp.55-63, Jun. 2009. google schola H. Y. Yu, I. J. Yang, V. R Lincha, I. S. Park, D. U. Lee, H. M. Shin, “The Effects of the Fruits of Foeniculum vulgare on Skin Barrier Function and Hyaluronic Acid Production in HaCaT Keratinocytes”, Journal of life science , Vol.25, No.8, pp.880-888, Agu. 2015. DOI: http://dx.doi.org/10.5352/JLS.2015.25.8.880 google schola H. S. Jung, M. Y. Song, H. S. Kim, H. H. Seo, J. H. Lee, K. R. Lee, I. Hong, S. H. Moh. “Development of Anti-Wrinkle Materials using Galloyl-Peptide Derivatives”, Journal of the Korea Academia-Industria , Vol.16, No.8, pp.5452-5457, Agu. 2015. DOI: http://dx.doi.org/10.5762/KAIS.2015.16.8.5452 google schola M. A. Ruiz, B. Clares, M. E. Morales, V. Gallardo. “Evaluation of the anti-wrinkle efficacy of cosmetic formulations with an anti-aging peptide (Argireline®)”, ARS Pharmaceutica , Vol.50, No.4, pp.168-176, Nov. 2009. google schola

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