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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.4
- 수록면
- 482 - 482 (1page)
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Infection with dengue virus (DENV) is a serious health issue that causes severe dengue fever and occasionally lethal complications, such as dengue hemorrhagic fever. Therefore, rapid and sensitive DENV detection is important to reduce morbidity and mortality. Here, we screened dengue NS1-specific affibodies and developed anti-NS1 affibody-functionalized AuNPs (anti-NS1 affibody-AuNPs) to improve the detection sensitivity of NS1 antigen in ELISA. First, we screened NS1 antigen-specific affibody molecules (ZNS112, ZNS116, and ZNS146) from the affibody phage library. The affibodies were then expressed and purified from Escherichia coli. Among them, the ZNS112 affibody showed the highest equilibrium binding constant (Kd) of 1 μM and this affibody was functiona ... 전체 초록 보기
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