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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.4
- 수록면
- 557 - 557 (1page)
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The 3-hydroxypropionic acid (3-HP) tolerance in Klebsiella pneumoniae was tested by growing the cell with glycerol as carbon source in presence of varied initial 3-HP addition, which was determined based on the 3-HP tolerance level of E. coli. The transcription factor, yieP, was studied carefully and it has been observed as gene regulating yohJK, the putative 3-HP exporter encoding gene in E. coli [1, 2]. We examined the homologous gene in K. pneumoniae which has similar genomic identity compared with the yieP and yohJK in E. coli by using sequence alignment and found that there are one and two identical genes for yieP and yohJK, respectively. We tested the deletion mutant of K. pneumoniae J2B for yieP, yohJK1, and yohJK2 to check the possibility of same functional role from the gene. The yieP deletion mutant strain showed much better growth profile than wild type when the initial 3-HP concentration is higher than 300mM up to 500mM; the yohJK1 deletion mutant along with yieP deletion showed highly reduced tolerance compared with yieP single deletion, but yohJK2 deletion mutant showed similar gro ... 전체 초록 보기
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