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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.5
- 수록면
- 2 - 2 (1page)
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Background: This study was carried out that development of HPLC validation for analysis of main cannabinoids (CBD, CBDA Δ<SUP>9</SUP>-THC, and Δ<SUP>9</SUP>-THCA) on inflorescence of hemp (Cannabis sativa L.) in a short time. CBD is the main medicinal component of cannabis, and Δ<SUP>9</SUP>-THC is a psychotropic component was originated from acidic cannabinoids (CBDA and Δ<SUP>9</SUP>-THCA) through non-enzyme decarboxylation. New analysis method applied, the characteristics of decarboxylation of raw material such as inflorescence were identified by converting acid cannabinoids into neutral cannabinoids that are active in the human body.
Methods and Results: UV spectrum of each of the four common cannabinoids were scanned and the cut-off section of the mobile solvent (MeOH, ACN, Water) was identified without interference. Uniform elution of acidic cannabinoids (CBDA, Δ<SUP>9</SUP>-THCA) without peak tailing, acids (phosphoric acid, formic acid, trifluoroacetic acid) were tested. The established conditions were validated with system suitability test, linearity, precision, detection limit, and quantitation limit. Inflorescence of hemp was feeze-dried and pulverized by twelve mesh, and decarboxylation process was pe ... 전체 초록 보기
Methods and Results: UV spectrum of each of the four common cannabinoids were scanned and the cut-off section of the mobile solvent (MeOH, ACN, Water) was identified without interference. Uniform elution of acidic cannabinoids (CBDA, Δ<SUP>9</SUP>-THCA) without peak tailing, acids (phosphoric acid, formic acid, trifluoroacetic acid) were tested. The established conditions were validated with system suitability test, linearity, precision, detection limit, and quantitation limit. Inflorescence of hemp was feeze-dried and pulverized by twelve mesh, and decarboxylation process was pe ... 전체 초록 보기
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