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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제2호
- 발행연도
- 2021.1
- 수록면
- 280 - 289 (10page)
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초록· 키워드
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Genetic markers currently used for the discrimination of Lactobacillus delbrueckii subspecies have low efficiency for identification at subspecies level. Therefore, our objective in this study was to select novel genetic markers for accurate identification and discrimination of six L. delbrueckii subspecies based on pangenome analysis. We evaluated L. delbrueckii genomes to avoid making incorrect conclusions in the process of selecting genetic markers due to mislabeled genomes. Genome analysis showed that two genomes of L. delbrueckii subspecies deposited at NCBI were misidentified. Based on these results, subspecies-specific genetic markers were selected by comparing the core and pangenomes. Genetic markers were confirmed to be specific for 59,196,562 genome sequences via in silico analysis. They were found in all strains of the same subspecies, but not in other subspecies or bacterial strains. These genetic markers also could be used to accurately identify genomes at the subspecies level for genomes known at the species level. A real-time PCR method for detecting three main subspecies (L. delbrueckii subsp. delbrueckii, lactis, and bulgaricus) was developed to cost-effectively identify them using genetic markers. Results showed 100% specificity for each subspecies. These genetic markers could differentiate each subspecies from 44 other lactic acid bacteria. This real-time PCR method was then applied to monitor 26 probiotics and dairy products. It was also used to identify 64 unknown strains isolated from raw milk samples and dairy products. Results confirmed that unknown isolates and subspecies contained in the product could be accurately identified using this real-time PCR method.
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참고문헌 (31)
참고문헌 신청
El Kafsi H / 2014 / Lactobacillus delbrueckii ssp. lactis and ssp. bulgaricus:A chronicle of evolution in action / BMC. Genomics 15:407
Zanni E / 2017 / Combination of metabolomic and proteomic analysis revealed different features among Lactobacillus delbrueckii subspecies bulgaricus and lactis strains while in vivo testing in the model organism Caenorhabditis elegans highlighted probiotic properties / Front. Microbiol 8:1206
van De Guchte M / 2006 / The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution / Proc. Natl. Acad. Sci. USA 103:9274~9279
Nikolaou A / 2011 / A new methodology for rapid detection of Lactobacillus delbrueckii subsp. bulgaricus based on multiplex PCR / J. Microbiol. Methods 84:362~364
Weiss N / 1983 / Lactobacillus lactis, Lactobacillus leichmannii and Lactobacillus bulgaricus, subjective synonyms of Lactobacillus delbrueckii, and description of Lactobacillus delbrueckii subsp. lactis comb. nov. and Lactobacillus delbrueckii subsp. bulgaricus comb. nov / Syst. Appl. Microbiol 4:552~557
Zanni E / 2017 / Combination of metabolomic and proteomic analysis revealed different features among Lactobacillus delbrueckii subspecies bulgaricus and lactis strains while in vivo testing in the model organism Caenorhabditis elegans highlighted probiotic properties / Front. Microbiol 8:1206
van De Guchte M / 2006 / The complete genome sequence of Lactobacillus bulgaricus reveals extensive and ongoing reductive evolution / Proc. Natl. Acad. Sci. USA 103:9274~9279
Nikolaou A / 2011 / A new methodology for rapid detection of Lactobacillus delbrueckii subsp. bulgaricus based on multiplex PCR / J. Microbiol. Methods 84:362~364
Weiss N / 1983 / Lactobacillus lactis, Lactobacillus leichmannii and Lactobacillus bulgaricus, subjective synonyms of Lactobacillus delbrueckii, and description of Lactobacillus delbrueckii subsp. lactis comb. nov. and Lactobacillus delbrueckii subsp. bulgaricus comb. nov / Syst. Appl. Microbiol 4:552~557
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