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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제31권 제7호
- 발행연도
- 2021.1
- 수록면
- 903 - 911 (9page)
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Previous studies have modified microbial genomes by introducing gene cassettes containing selectable markers and homologous DNA fragments. However, this requires several steps including homologous recombination and excision of unnecessary DNA regions, such as selectable markers from the modified genome. Further, genomic manipulation often leaves scars and traces that interfere with downstream iterative genome engineering. A decade ago, the CRISPR/Cas system (also known as the bacterial adaptive immune system) revolutionized genome editing technology. Among the various CRISPR nucleases of numerous bacteria and archaea, the Cas9 and Cas12a (Cpf1) systems have been largely adopted for genome editing in all living organisms due to their simplicity, as they consist of a single polypeptide nuclease with a target-recognizing RNA. However, accurate and fine-tuned genome editing remains challenging due to mismatch tolerance and protospacer adjacent motif (PAM)-dependent target recognition. Therefore, this review describes how to overcome the aforementioned hurdles, which especially affect genome editing in higher organisms. Additionally, the biological significance of CRISPR-mediated microbial genome editing is discussed, and future research and development directions are also proposed.
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참고문헌 (4)
참고문헌 신청
Sang-Hyun Lee / 2016 / Single Crossover-Mediated Markerless Genome Engineering in Clostridium acetobutylicum / Journal of Microbiology and Biotechnology 26(4):725~729
이남일 / 2019 / Synthetic Biology Tools for Novel Secondary Metabolite Discovery in Streptomyces / Journal of Microbiology and Biotechnology 29(5):667~686
김범준 / 2020 / Effective Blocking of Microbial Transcriptional Initiation by dCas9-NGMediated CRISPR Interference / Journal of Microbiology and Biotechnology 30(12):1919~1926
김현주 / 2020 / Single-Base Genome Editing in Corynebacterium glutamicum with the Help of Negative Selection by Target-Mismatched CRISPR/Cpf1 / Journal of Microbiology and Biotechnology 30(10):1583~1591
이남일 / 2019 / Synthetic Biology Tools for Novel Secondary Metabolite Discovery in Streptomyces / Journal of Microbiology and Biotechnology 29(5):667~686
김범준 / 2020 / Effective Blocking of Microbial Transcriptional Initiation by dCas9-NGMediated CRISPR Interference / Journal of Microbiology and Biotechnology 30(12):1919~1926
김현주 / 2020 / Single-Base Genome Editing in Corynebacterium glutamicum with the Help of Negative Selection by Target-Mismatched CRISPR/Cpf1 / Journal of Microbiology and Biotechnology 30(10):1583~1591
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