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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제4호
- 발행연도
- 2016.1
- 수록면
- 725 - 729 (5page)
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초록· 키워드
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A novel genome-engineering tool in Clostridium acetobutylicum was developed based on singlecrossover homologous recombination. A small-sized non-replicable plasmid, pHKO1, was designed for efficient integration into the C. acetobutylicum genome. The integrated pHKO1 plasmid backbone, which included an antibiotic resistance gene, can be excised in vivo by Flp recombinase, leaving a single flippase recognition target sequence in the middle of the targeted gene. Since the pSHL-FLP plasmid, the carrier of the Flp recombinase gene, employed the segregationally unstable pAMβ1 replicon, the plasmid was rapidly cured from the mutant C. acetobutylicum. Consequently, our method makes it easier to engineer C. acetobutylicum.
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참고문헌 (19)
참고문헌 신청
Al-Hinai MA / 2012 / Novel system for efficient isolation of Clostridium double-crossover allelic exchange mutants enabling markerless chromosomal gene deletions and DNA integration / Appl. Environ. Microbiol 78:8112~8121
Awad MM / 1995 / Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringensmediated gas gangrene / Mol. Microbiol 15:191~202
Bi C / 2011 / SpoIIE is necessary for asymmetric division, sporulation, and expression of σF, σE, and σG but does not control solvent production in Clostridium acetobutylicum ATCC 824 / J. Bacteriol 193:5130~5137
Cartman ST / 2012 / Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production / Appl. Environ. Microbiol 78:4683~4690
Green EM / 1996 / Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824 / Microbiology 142:2079~2086
Awad MM / 1995 / Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringensmediated gas gangrene / Mol. Microbiol 15:191~202
Bi C / 2011 / SpoIIE is necessary for asymmetric division, sporulation, and expression of σF, σE, and σG but does not control solvent production in Clostridium acetobutylicum ATCC 824 / J. Bacteriol 193:5130~5137
Cartman ST / 2012 / Precise manipulation of the Clostridium difficile chromosome reveals a lack of association between the tcdC genotype and toxin production / Appl. Environ. Microbiol 78:4683~4690
Green EM / 1996 / Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824 / Microbiology 142:2079~2086
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