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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.1
- 수록면
- 151 - 161 (11page)
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Wheat (Triticum aestivum L.) is a major food crop worldwide whose complex and very large genome hinders stable genetic transformation. An optimized method for transformation by particle bombardment is therefore greatly needed, both in general and for Korean commercial wheat varieties, which have not yet been successfully transformed. In this study, we describe the stable transformation of the Korean commercial wheat variety Keumkang and the DH20, which lacks the Glu-B3 and Gli?B1 loci (both related to gluten production), via particle bombardment of a vector harboring the enhanced green fuorescent protein (eGFP) gene. We confrmed the insertion of the eGFP transgene into the genome of primary (T0) transformants by genomic PCR, RT-qPCR, and fuorescence imaging. Our estimated average transformation efciencies were 4.4% for Keumkang and 3.5% for DH20 in the T0 generation. We also validated the stable expression of the transgene in the T1 gen?eration. We hypothesize that donor plant health, centrifugation, and high-sucrose pre-treatment increased the transformation efciency reported here. Taken together, these results describe a useful method for transforming Korean commercial wheat varieties, including Keumkang and DH20, that will allow the manipulation of gene expression via such techniques as RNA interference, overexpression, and genome editing.
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참고문헌 (49)
참고문헌 신청
Altenbach SB / 2011 / Transformation of the US bread wheat ‘Butte 86’ and silencing of omega-5 gliadin genes / GM Crops 2:66~73
Altpeter F / 1996 / Accelerated production of transgenic wheat (Triticum aestivum L.) plants / Plant Cell Rep 16:12~17
Arumuganathan K / 1991 / Estimation of nuclear DNA content of plants by flow cytometry / Plant Mol Biol Rep 9:229~241
Barro F / 1997 / Transformation of wheat with highmolecular- weight subunit genes results in improved functional properties / Nat Biotechnol 15:1295~1299
Blechl AE / 1996 / Expression of a novel high-molecularweight glutenin subunit gene in transgenic wheat / Nat Biotechnol 14:875~879
Altpeter F / 1996 / Accelerated production of transgenic wheat (Triticum aestivum L.) plants / Plant Cell Rep 16:12~17
Arumuganathan K / 1991 / Estimation of nuclear DNA content of plants by flow cytometry / Plant Mol Biol Rep 9:229~241
Barro F / 1997 / Transformation of wheat with highmolecular- weight subunit genes results in improved functional properties / Nat Biotechnol 15:1295~1299
Blechl AE / 1996 / Expression of a novel high-molecularweight glutenin subunit gene in transgenic wheat / Nat Biotechnol 14:875~879
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