In vivo tumour imaging employing regional delivery of novel Gallium radiolabelled polymer composites

Ross W. Stephens; Gregory D. Tredwell; Jessica L. Bell; Karen J. Knox; Lee A. Philip; Tim J. Senden; Michael J. Tapner; Stephanie A. Bickley; Marcel R. Tanudji; Stephen K. Jones

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자료유형: 학술저널

저자정보: Ross W. Stephens (Department of Applied Mathematics Australian National University Australia) Gregory D. Tredwell (Department of Applied Mathematics Australian National University Australia) Jessica L. Bell (Department of Applied Mathematics Australian National University Australia) Karen J. Knox (Department of Applied Mathematics Australian National University Australia) Lee A. Philip (Department of Applied Mathematics Australian National University Australia) Tim J. Senden (Department of Applied Mathematics Australian National University Australia) Michael J. Tapner (Sirtex Medical Ltd Sydney Australia) Stephanie A. Bickley (Sirtex Medical Ltd Sydney Australia) Marcel R. Tanudji (Sirtex Medical Ltd Sydney Australia) Stephen K. Jones (Sirtex Medical Ltd Sydney Australia)

저널정보: 한국생체재료학회 생체재료학회지 생체재료학회지 제25권 제2호

발행연도: 2021.1

수록면: 147 - 155 (9page)

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Background: Understanding the regional vascular delivery of particles to tumour sites is a prerequisite for developing new diagnostic and therapeutic composites for treatment of oncology patients. We describe a novel imageable 67Ga-radiolabelled polymer composite that is biocompatible in an animal tumour model and can be used for preclinical imaging investigations of the transit of different sized particles through arterial networks of normal and tumour-bearing organs. Results: Radiolabelling of polymer microspheres with 67Ga was achieved using a simple mix and wash method, with tannic acid as an immobilising agent. Final in vitro binding yields after autoclaving averaged 94.7%. In vivo stability of the composite was demonstrated in New Zealand white rabbits by intravenous administration, and intrahepatic artery instillations were made in normal and VX2 tumour implanted rabbit livers. Stability of radiolabel was sufficient for rabbit lung and liver imaging over at least 3 hours and 1 hour respectively, with lung retention of radiolabel over 91%, and retention in both normal and VX2 implanted livers of over 95%. SPECT-CT imaging of anaesthetised animals and planar imaging of excised livers showed visible accumulation of radiolabel in tumours. Importantly, microsphere administration and complete liver dispersal was more easily achieved with 8 μm diameter MS than with 30 μm MS, and the smaller microspheres provided more distinct and localised tumour imaging. Conclusion: This method of producing 67Ga-radiolabelled polymer microspheres is suitable for SPECT-CT imaging of the regional vascular delivery of microspheres to tumour sites in animal models. Sharper distinction of model tumours from normal liver was obtained with smaller MS, and tumour resolution may be further improved by the use of 68Ga instead of 67Ga, to enable PET imaging.

#Vascular particle delivery #Tumour plexus #Tumour imaging #Radiolabelled polymer microspheres #Rabbit tumour model

참고문헌 (22)

참고문헌 신청

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