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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 85 - 92 (8page)
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초록· 키워드
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A γ-aminobutyric acid (GABA) producing microorganism was isolated from Sun-Tae Jeotgal, a Korean traditional fermented seafood. Two thousand presumptive lactic acid bacteria (LAB) isolates were screened for GABA production by thin layer chromatography. One isolate, T118, produced GABA profusely, and identified as Lactobacillus brevis. Growth of Lb. brevis T118 was examined during 120 h cultivation in MRS broth under different conditions. Lb. brevis T118 grew well at 30-37℃, initial pH of 4-7, and up to 5% NaCl (w/v). A gene, gadB, encoding glutamate decarboxylase (GAD) was cloned by PCR. gadC encoding a glutamate/GABA antiporter was cloned and gadC located immediately upstream of gadB, indicating gadCB operon structure. The operon structure was confirmed by reverse transcription (RT)-PCR. gadB was overexpressed in Escherichia coli BL21 (DE3) and recombinant GAD was purified. The size of recombinant GAD was 54.4 kDa by SDS-PAGE, which matched well with the calculated size from the nucleotide sequence.
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참고문헌 (20)
참고문헌 신청
Coton E / 2009 / Evidence of horizontal transfer as origin of strain to strain variation of the tyramine production trait in Lactobacillus brevis / Food Microbiol 26:52~57
Fan E / 2012 / Cloning, sequencing and expression of a glutamate decarboxylase gene from the GABA-producing strain Lactobacillus brevis CGMCC 1306 / Ann. Microbiol 62:689~698
Hiraga K / 2008 / Glutamate decarboxylase from Lactobacillus brevis: Activation by ammonium sulfate / Biosci. Biotechnol. Biochem 72:1299~1306
Komatsuzaki N / 2008 / Characterization of glutamate decarboxylase from a high γ-aminobutyric acid (GABA)-producer, Lactobacillus paracasei / Biosci. Biotechnol. Biochem. 72:278~285
Le Vo TD / 2013 / Efficient gamma-aminobutyric acid bioconversion by employing synthetic complex between glutamate decarboxylase and glutamate/GABA antiporter in engineered Escherichia coli / J. Ind. Microbiol. Biotechnol 40:927~933
Fan E / 2012 / Cloning, sequencing and expression of a glutamate decarboxylase gene from the GABA-producing strain Lactobacillus brevis CGMCC 1306 / Ann. Microbiol 62:689~698
Hiraga K / 2008 / Glutamate decarboxylase from Lactobacillus brevis: Activation by ammonium sulfate / Biosci. Biotechnol. Biochem 72:1299~1306
Komatsuzaki N / 2008 / Characterization of glutamate decarboxylase from a high γ-aminobutyric acid (GABA)-producer, Lactobacillus paracasei / Biosci. Biotechnol. Biochem. 72:278~285
Le Vo TD / 2013 / Efficient gamma-aminobutyric acid bioconversion by employing synthetic complex between glutamate decarboxylase and glutamate/GABA antiporter in engineered Escherichia coli / J. Ind. Microbiol. Biotechnol 40:927~933
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