지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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The nitrilase-like (NIT) protein subfamily is a member of the nitrilase superfamily, which exhibits CN hydrolase activity. NIT proteins are highly conserved, and mammals have Nit1 and Nit2. Nit proteins are putative tumor suppressors; however, the enzymatic activities of Nit1 and Nit2 are different. Nit1 is a metabolite repair enzyme, whereas Nit2 is an ω-amidase. In this study, in order to understand the structural features and functional diversity of Nit proteins, Nit2 from Kluyveromyces lactis was crystallized, and X-ray diffraction data were obtained at a resolution of 2.2 A. The crystal of K. lactis Nit2 (KlNit2) belonged to the space group C2, with the unit cell parameters a = 79.0 A, b = 211.1 A, c = 89.4 A, α = γ = 90°, β = 112.1°. The crystal contained four macromolecules in the asymmetric unit.
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참고문헌 (13)
참고문헌 신청
Adams, P. D. / 2010 / PHENIX : a comprehensive Python-based system for macromolecular structure solution / Acta Crystallogr D Biol Crystallogr 66:213~221
Barglow, K. T. / 2008 / Functional proteomic and structural insights into molecular recognition in the nitrilase family enzymes / Biochemistry 47:13514~13523
de Crécy-Lagard, V. / 2018 / Newly-discovered enzymes that function in metabolite damage-control / Curr Opin Chem Biol 47:101~108
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Jaisson, S. / 2009 / Molecular identification of omega-amidase, the enzyme that is functionally coupled with glutamine transaminases, as the putative tumor suppressor Nit2 / Biochimie 91:1066~1071
Barglow, K. T. / 2008 / Functional proteomic and structural insights into molecular recognition in the nitrilase family enzymes / Biochemistry 47:13514~13523
de Crécy-Lagard, V. / 2018 / Newly-discovered enzymes that function in metabolite damage-control / Curr Opin Chem Biol 47:101~108
Emsley, P. / 2004 / Coot : model-building tools for molecular graphics / Acta Crystallogr D Biol Crystallogr 60:2126~2132
Jaisson, S. / 2009 / Molecular identification of omega-amidase, the enzyme that is functionally coupled with glutamine transaminases, as the putative tumor suppressor Nit2 / Biochimie 91:1066~1071
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