Kinesin Family Member 11 Enhances the Self-Renewal Ability of Breast Cancer Cells by Participating in the Wnt/β-Catenin Pathway

Yuan-yuan Pei; Gao-chi Li; Jian Ran; Xin-hong Wan; Feng-xiang Wei; Lan Wang

추천

검색

자료유형: 학술저널

저자정보: Yuan-yuan Pei (Shenzhen Long-gang Maternal and Child Health Hospital Centralab Shenzhen China) Gao-chi Li (Shenzhen Long-gang Maternal and Child Health Hospital Centralab Shenzhen China) Jian Ran (Shenzhen Long-gang Maternal and Child Health Hospital Centralab Shenzhen China) Xin-hong Wan (Shenzhen Long-gang Maternal and Child Health Hospital Centralab Shenzhen China) Feng-xiang Wei (Shenzhen Long-gang Maternal and Child Health Hospital Centralab Shenzhen China) Lan Wang (Department of Pathogen Biology and Immunology School of Life Sciences and Biopharmaceutics Guangdo)

저널정보: 한국유방암학회 Journal of Breast Cancer Journal of Breast Cancer Vol.22 No.4

발행연도: 2019.1

수록면: 522 - 532 (11page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Purpose: Our previous studies have shown that kinesin family member 11 (KIF11) is markedly overexpressed in human breast cancer cells or tissues and positively correlated with distant metastasis and prognosis in patients with breast cancer, suggesting an important role in the regulation of cancer stem cells. Herein, we examined the role of KIF11 in breast cancer stem cells. Methods: In the current study, we validated our previous findings through analysis of data collected in The Cancer Genome Atlas. Endogenous KIF11 was stably silenced in MCF-7 and SKBR-3 cells. Flow cytometry was used to measure the proportion of side-population (SP) cells. Mammosphere culture and tumor implantation experiments in immunodeficient mice were used to assess the self-renewal ability of breast cancer cells. Real-time polymerase chain reaction, western blot, immunofluorescence staining, luciferase reporter assays and Wnt agonist treatment were conducted to investigate the signaling pathways regulated by KIF11. Results: We found that the expression level of KIF11 was positively correlated with stem cell-enrichment genes. The proportion of SP cells was significantly reduced in KIF11-silenced cells. Silencing endogenous KIF11 not only reduced the size and number of mammospheres in vitro, but also reduced the ability of breast cancer cells to form tumors in mice. Simultaneously, we found that KIF11 was involved in regulating the activation of the Wnt/β-catenin signaling pathway. Conclusion: Endogenous KIF11 enhances the self-renewal of breast cancer cells by activating the Wnt/β-catenin signaling pathway, thereby enhancing the characteristics of breast cancer stem cells.

#Breast neoplasms #Kinesin #Cell self renewal #Stem cells #Beta-catenin

참고문헌 (28)

참고문헌 신청

Yap YS / 2019 / Insights into breast cancer in the East vs the West : a review / JAMA Oncol 5:1489~1496

Gonzalez ME / 2017 / Mesenchymal stem cell-induced DDR2 mediates stromal-breast cancer interactions and metastasis growth / Cell Reports 18:1215~1228

Zhang GF / 2016 / Cancer stem cell targets-a review / Eur Rev Med Pharmacol Sci 20:2045~2051

Tsang JY / 2012 / Cancer stem cell markers are associated with adverse biomarker profiles and molecular subtypes of breast cancer / Breast Cancer Res Treat 136:407~417

Kasimir-Bauer S / 2012 / Expression of stem cell and epithelial-mesenchymal transition markers in primary breast cancer patients with circulating tumor cells / Breast Cancer Res 14:R15

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!