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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 208 - 219 (12page)
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초록· 키워드
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High-throughput molecular markers with high genotyping accuracy will be helpful for genetic analysis, mapping of interesting genes, and rice breeding program. To develop high-throughput and cost-effective molecular markers for Korean japonica rice varieties, which are closely-related genetically, we designed kompetitive allele-specific polymerase chain reaction (KASP) assays from the sequence data of 13 Korean japonica rice varieties. Of the 504 new KASP assays, 371 (73.6%) showed polymorphisms among the tested varieties. In addition to the 400 previously developed KASP markers, this resulted in 771 KASP markers being applicable for Korean japonica rice varieties. These KASP markers were used to map the quantitative trait loci (QTLs) for rice bakanae disease (BD) resistance. From the results of QTL mapping and determination of the mortality rate of BD in two F2:F3 populations, a major QTL, qFfR1-1, and a novel QTL, qFfR6, were revealed on chromosome 1 in the Junam/Nampyeong F2:F3 population and on chromosome 6 in the Saenuri/Nampyeong F2:F3 population, respectively. Further, the insertion/deletion markers in the qFfR1-1 region were developed to select BD-resistant japonica rice varieties. The 771 developed KASP markers will accelerate the molecular breeding in Korean japonica rice varieties, and the detected QTLs will be helpful in identifying candidate genes for BD resistance.
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등록된 정보가 없습니다.
참고문헌 (30)
참고문헌 신청
Basten CJ / 1996 / QTL cartographer : a reference manual and tutorial for QTL mapping
천경성 / 2018 / Single Nucleotide Polymorphism (SNP) Discovery and Kompetitive Allele-Specific PCR (KASP) Marker Development with Korean Japonica Rice Varieties / Plant Breeding and Biotechnology 6(4):391~403
Desjardins AE / 2000 / Fusarium species from Nepalese rice and production of mycotoxins and gibberellic acid by selected species / Appl. Environ. Microbiol 66:1020~1025
Fiyaz RA / 2016 / Mapping quantitative trait loci responsible for resistance to Bakanae disease in rice / Rice 9:45
Gao LZ / 2008 / Nonindependent domestication of the two rice subspecies, Oryza sativa ssp. Indica and ssp. Japonica, demonstrated by multilocus microsatellites / Genetics 179:965~976
천경성 / 2018 / Single Nucleotide Polymorphism (SNP) Discovery and Kompetitive Allele-Specific PCR (KASP) Marker Development with Korean Japonica Rice Varieties / Plant Breeding and Biotechnology 6(4):391~403
Desjardins AE / 2000 / Fusarium species from Nepalese rice and production of mycotoxins and gibberellic acid by selected species / Appl. Environ. Microbiol 66:1020~1025
Fiyaz RA / 2016 / Mapping quantitative trait loci responsible for resistance to Bakanae disease in rice / Rice 9:45
Gao LZ / 2008 / Nonindependent domestication of the two rice subspecies, Oryza sativa ssp. Indica and ssp. Japonica, demonstrated by multilocus microsatellites / Genetics 179:965~976
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